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为了确定酸解预处理秸秆还原糖产量较高的玉米生育时期,为乙醇发酵生产提供较优原料,以“渝单8号”玉米为材料,采用DNS法测定不同稀硫酸预处理和酶解条件下拔节期、抽雄期、抽丝期、乳熟期、成熟期秸秆的总还原糖产量。对水解温度、水解时间、稀硫酸体积分数、固液质量比进行单因素试验,再通过正交试验优化预处理条件。结果表明:在稀硫酸预处理条件下,抽丝期的玉米秸秆总还原糖产量较高,更适合乙醇发酵生产;在处理温度110℃、处理时间40 m in、硫酸体积分数2%、固液质量比1∶15的最优预处理条件下,还原糖产量达549.42 mg/g。
In order to determine the optimal growth period of maize with high yield of straw reducing sugar by acid hydrolysis pretreatment, the optimal raw material for ethanol fermentation was provided. The “Danyun No.8” maize was used as raw material to determine the effect of different dilute sulfuric acid pretreatment and enzyme Under the conditions of jointing, tasseling, drawing period, milky maturity, maturity of the total reducing sugar production. The hydrolysis temperature, hydrolysis time, the volume fraction of dilute sulfuric acid, solid-liquid mass ratio of single factor test, and then through orthogonal test to optimize the pretreatment conditions. The results showed that under the condition of dilute sulfuric acid pretreatment, the yield of total reducing sugars of corn straw during spinning was higher, which was more suitable for the ethanol fermentation. When the treatment temperature was 110 ℃, the treatment time was 40 min, the volume fraction of sulfuric acid was 2% Under optimal pretreatment conditions, the yield of reducing sugar reached 549.42 mg / g.