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本研究探讨T细胞急性淋巴细胞白血病(T-ALL)免疫表型特点。应用一组系列相关单克隆抗体和CD45/SSC设门的三色流式细胞术对140例T-ALL进行免疫表型检测。结果显示,白血病细胞表面T系抗原表达依次为CD7>CD2>CD3>CD5,近20%患者CD10表达阳性。140例患者中12例带有B系抗原标记,阳性率达8.57%;136例中31例带有髓系抗原标记,阳性率达22.79%,所有病例均无CD14表达。干/祖细胞标记CD34表达阳性率31.06%,41例CD34+T-ALL髓系抗原表达15例,阳性率36.59%,91例CD34-T-ALL髓系抗原表达14例,阳性率15.38%,二者具有统计学差异(p<0.01)。儿童T-ALLCD3表达高于成人(p<0.05),而CD33表达明显低于成人(p<0.01)结论:免疫表型分析是诊断T-ALL的重要手段,T-ALL免疫表型具有异质性。
This study was to investigate the immunophenotypic characteristics of T-cell acute lymphoblastic leukemia (T-ALL). A series of monoclonal antibodies and CD45 / SSC portal tri-color flow cytometry was used to detect the immunophenotype of 140 cases of T-ALL. The results showed that the expression of T-antigen on the surface of leukemia cells was CD7> CD2> CD3> CD5, and nearly 10% of the patients showed positive CD10 expression. Of the 140 patients, 12 cases were labeled with B lineage antigen, the positive rate was 8.57%. Among 136 cases, 31 cases were labeled with myeloid antigen with a positive rate of 22.79%. No CD14 expression was found in all cases. The positive rate of CD34 expression in stem / progenitor cells was 31.06%, in 15 cases of 41 CD34 + T-ALL myeloid antigens, the positive rate was 36.59% and in 14 cases of 91 cases of CD34-T-ALL myeloid antigens, the positive rate was 15.38% The difference was statistically significant (p <0.01). The expression of T-ALLCD3 in children was higher than that in adults (p <0.05), while the expression of CD33 was significantly lower than that in adults (p <0.01). Conclusion: Immunophenotyping is an important method to diagnose T-ALL. T-ALL immunophenotype is heterogeneous Sex.