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为了探讨自分泌运动因子(autocrine motility factor,AMF)对人胶质母细胞瘤U251细胞迁移、侵袭影响及其相关分子机制,该实验采用了RT-PCR及免疫印迹法检测RNA干扰AMF后U251细胞中AMF的表达变化;细胞划痕实验、Transwell实验分别观察了AMF干扰前后U251细胞迁移、侵袭能力的变化;免疫印记检测AMF干扰前后细胞中总Akt、p-Akt、Sox2、基质金属蛋白酶-2(matrix metalloprotein-2,MMP-2)及MMP-9蛋白水平的变化。研究结果表明,AMF成功干扰后U251细胞的迁移和侵袭能力受到抑制,p-Akt、Sox2、MMP-2和MMP-9蛋白表达水平降低。该研究表明,AMF敲低可以通过下调PI3K/Akt信号通路活性及Sox2、MMP-2和MMP-9蛋白水平,抑制人胶质母细胞瘤U251细胞迁移和侵袭。
In order to investigate the effect of autocrine motility factor (MIFF) on the migration and invasion of human glioblastoma U251 cells and its related molecular mechanisms, RT-PCR and Western blotting were used to detect U251 cells The changes of AMF expression were analyzed by cell scratch assay and Transwell assay. The changes of migration and invasion ability of U251 cells before and after AMF interference were observed. The expressions of Akt, p-Akt, Sox2 and MMP- (matrix metalloprotein-2, MMP-2) and MMP-9 protein levels. The results showed that the migration and invasion ability of U251 cells were inhibited after AMF interference, and the expressions of p-Akt, Sox2, MMP-2 and MMP-9 decreased. The study shows that AMF knockdown can inhibit the migration and invasion of human glioblastoma U251 cells by down-regulating the activity of PI3K / Akt signaling pathway and Sox2, MMP-2 and MMP-9 protein levels.