目的探讨狗组织相容性系统（ＤＬＡ）－ＤＲＢ１基因分型方法及用于狗卵巢移植配型的可行性。方法 选择特定的引物和 ６种限制性内切酶，采用聚合酶链反应－限制性片段长度多态性（ｐｏｌｙｍｅｒａｓｅ ｃｈａｉｎ　ｒｅａｃｔｉｏｎｂａｓｅｄｏｎｒｅｓｔｒｉｃｔｉｏｎｆｒａｇｍｅｎｔ　ｌｅｎｇｔｈ　ｐｏｌｙｍｏｒｐｈｉｓｍ， ＰＣＲ－ＲＦＬＰ）技术建立了 ＤＬＡ－ＤＲＢ１基因分型的方法。用 ＰＣＲ方法对 ６ 只无亲缘相关的个体纯种比格狗ＤＲＢ１基因扩增，通过选用 ＨａｅⅢ， Ｈｉｎｆ　Ⅰ， ＭｓｐⅠ， ＨｈａⅠ， Ｓａｕ９６Ⅰ及 ＲｓａⅠ等６种限制性内切酶对该片段进行酶切分析。结果酶切图谱显示出ＤＬＡ－ＤＲＢ１基因具有高度多态性。应用于３对卵巢移植狗的基因配型获得成功。结论研究表明，该方法在狗器官移植配型中有较好的实用价值。 OBJECTIVE To investigate the genotyping of dog histocompatibility system (DLA) -DRB1 and the feasibility of matching with dog ovary transplantation. Methods Specific primers and six restriction enzymes were selected and the DLA-DRB1 genotyping method was established by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). The DRB1 gene of six unrelated individuals was amplified by PCR. The fragment was digested with six restriction enzymes HaeⅢ, Hinf Ⅰ, MspⅠ, HhaⅠ, Sau96Ⅰ and RsaⅠ . Results The digestion pattern showed that the DLA-DRB1 gene was highly polymorphic. The genotyping applied to 3 pairs of ovarian transplantation dogs was successful. Conclusion The research shows that this method has good practical value in the matching of dog organ transplantation.