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目的:建立高效液相色谱法测定银杏总黄酮自乳化微乳中银杏总黄酮的含量。方法:采用菲罗门-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.4%磷酸溶液(50∶50,v/v)为流动相,流速为1.0 ml·min~(-1),检测波长为360 nm,柱温为30℃。结果:银杏总黄酮中槲皮素、山柰素和异鼠李素三种成分分离度较好,槲皮素在2.0~40.0μg·ml~(-1)质量浓度范围内,山柰素在3.0~60.0μg·ml~(-1)质量浓度范围内,异鼠李素在2.0~40.0μg·ml~(-1)质量浓度范围与峰面积呈良好的线性关系。槲皮素、山柰素和异鼠李素的平均回收率分别为98.4%,99.7%和100.5%,RSD分别为0.92%,0.62%和1.24%(n=9)。结论:该方法对银杏总黄酮自乳化微乳中三种成分分离效果较好,辅料无干扰,专属性强、稳定可靠,适用于银杏总黄酮自乳化微乳中银杏总黄酮的含量测定。
Objective: To establish a HPLC method for the determination of total flavonoids in Ginkgo biloba total flavone self-emulsifying microemulsion. METHODS: The mobile phase was Phenomenex-C 18 column (250 mm × 4.6 mm, 5 μm) with methanol-0.4% phosphoric acid (50:50, v / v) as the mobile phase at a flow rate of 1.0 ml · min- (-1), the detection wavelength was 360 nm and the column temperature was 30 ℃. Results: The separation of quercetin, kaempferol and isorhamnetin in the total flavonoids of Ginkgo biloba was better. The concentration of quercetin in the range of 2.0 ~ 40.0μg · ml ~ (-1) In the concentration range of 60.0μg · ml ~ (-1), isorhamnetin showed a good linear relationship with the peak area in the range of 2.0 ~ 40.0μg · ml ~ (-1) mass concentration. The average recoveries of quercetin, kaempferol and isorhamnetin were 98.4%, 99.7% and 100.5%, respectively, with RSDs of 0.92%, 0.62% and 1.24%, respectively (n = 9). Conclusion: This method has good effect on the separation of three components of total flavonoids in Ginkgo biloba, and has the advantages of no interference in accessories, strong specificity and stability, and is suitable for the determination of total flavonoids in Ginkgo biloba total flavone self-emulsifying microemulsion.