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目的为了解江干区散发和暴发腹泻病人、零售水产品中副溶血性弧菌的分布状况,以及不同来源副溶血性弧菌血清型及毒力基因的异同。方法根据GB/T4789.7-2008进行副溶血性弧菌的分离鉴定及血清学试验,并用荧光定量PCR对分离的菌株进行毒力基因tdh和trh的检测。结果散发、暴发腹泻病人,零售水产品副溶血性弧菌检出率分别为16.36%(45/275)、85.71%(18/21)、39.69%(102/257)。临床菌株(包括散发、暴发病人)主要血清群为O3、O1和O4,水产品菌株的主要血清群则集中在O1、O10、O2、O5和O11中。临床菌株的tdh、trh检出率分别为96.88%(62/64),1.56%(1/64),水产品菌株的tdh、trh均未检出。结论副溶血性弧菌是江干区散发和暴发腹泻病人的主要致病菌,零售水产品受副溶血性弧菌污染严重,临床来源与水产品来源菌株在血清群分布及毒力基因携带有很大差异。
Objective To understand the distribution of Vibrio parahaemolyticus in retail and outbreak of diarrhea patients and retail aquatic products in Jianggan District, as well as the similarities and differences of serotypes and virulence genes of Vibrio parahaemolyticus from different sources. Methods Isolation and identification of Vibrio parahaemolyticus and serological tests were carried out according to GB / T4789.7-2008, and the virulence genes tdh and trh were detected by fluorescence quantitative PCR. The results showed that the detection rate of Vibrio parahaemolyticus was 16.36% (45/275), 85.71% (18/21) and 39.69% (102/257), respectively. The major serogroups of clinical strains (including sporadic outbreaks) were O3, O1 and O4, while the major serogroups of aquatic strains were concentrated in O1, O10, O2, O5 and O11. The detection rates of tdh and trh in clinical isolates were 96.88% (62/64) and 1.56% (1/64), respectively, and none of tdh and trh in aquatic products was detected. Conclusion Vibrio parahaemolyticus is the main pathogen of diarrhea in Jianggan district. The retail aquatic products are seriously polluted by Vibrio parahaemolyticus. The clinical sources and aquatic strains are highly distributed in serogroups and virulence genes Big difference.