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目的动态观察乳酸杆菌制剂对应用抗生素大鼠肠道菌群结构和TLR2 mRNA转录水平的影响。方法采用细菌培养法定量检测肠道双歧杆菌、乳酸杆菌、肠杆菌和肠球菌;利用反转录聚合酶链反应技术测定大鼠肠黏膜组织、肠系膜淋巴结、肝脏和脾脏细胞TLR2 mRNA转录水平。结果应用抗生素可致肠道菌群失调和TLR2 mRNA转录水平的早期受抑制。乳酸杆菌制剂干预可迅速提高肠道乳酸杆菌数量,及早扶正肠道菌群结构,减轻由于应用抗生素引起的Toll样受体mRNA转录受抑程度。结论乳酸杆菌制剂早期干预可及早扶正肠道菌群结构,减轻TLR2 mRNA转录水平受抑制程度,为临床合理应用抗生素,早期益生菌干预提供理论依据。
Objective To dynamically observe the effects of lactobacillus on the intestinal microflora and TLR2 mRNA transcription level in antibiotic-resistant rats. Methods Bifidobacteria, Lactobacilli, Enterobacteriaceae and Enterococcus were quantitatively detected by bacterial culture. The transcription level of TLR2 mRNA in intestinal mucosa, mesenteric lymph nodes, liver and spleen of rats was determined by reverse transcription polymerase chain reaction. Results The application of antibiotics can cause intestinal flora imbalance and early inhibition of TLR2 mRNA transcription. Intervention of lactobacillus can rapidly increase the number of Lactobacillus in the intestine and early rectify the structure of intestinal flora, alleviating the inhibition of Toll-like receptor mRNA transcription by antibiotics. Conclusion Early intervention with Lactobacillus can improve the structure of intestinal flora early and reduce the inhibition of TLR2 mRNA transcription level, providing a theoretical basis for rational use of antibiotics and early probiotics in clinics.