Large animal models are essential to pre-clinical trials of pulmonary transplantation and bronchial anastomosis poses a great technical challenge to the procedure.Presented here is a simplified continuous two-stitch suture technique into bronchial anastomosis during the course of left single lung transplantation in canine.Animals were divided into three groups with each group having 6 animals.Left single lung transplantation in canine was performed to assess the feasibility of using this technique for bronchial anastomosis.In the control groups,all anastomoses were done by using traditional technique.Allograft functions and hemodynamic parameters were monitored during a 3-h reperfusion period.Quality of bronchial healing and airway complications were assessed by bronchoscopic surveillance after transplantation.We successfully completed left lung transplantation in 18 dogs,and all the dogs survived the procedures.The new technique substantially simplified the procedures for bronchial anastomosis and greatly reduced the time for bronchial anastomosis(P＜0.01)and the ischemic time of the grafts(P＜0.05)compared to the control group.The continuous two-stitch suture attenuated the tissue injury to allografts and led to better blood gas exchange function as compared to the control group(P＜0.05).Good bronchial healing(Grade Ⅰ)was observed in all the groups.A canine left single lung transplantation model is feasible by using the novel suture technique,and the new technique is as safe as the traditional method.The technique is easy to learn,particularly for less experienced operators.Simpler and time-saving,the technique has great potential to be widely employed in clinical lung transplantation.