本文应用 ELISA-双抗体夹心法,通过检出血中的人 IgG 鉴定人血痕。双抗体夹心法是常用来检测抗原的一种方法,但在法医学上用于测定血痕种属尚少报道。我们建立的这种方法,新鲜人血痕的阳性结果可测到64万倍。保存三年的陈旧血痕仍可测出。马、牛、羊、狗、猪、鸡、鸭、鸽、兔、驴、骡和鹌鹑均为阴性。由于本法灵敏度高、特异性好、试剂易得,勿须贵重仪器,在物证检验中便于推广。 In this paper, ELISA-double antibody sandwich method, by detecting human IgG in blood to identify human bloodstream. Diabody sandwich method is commonly used to detect antigens, but in forensic medicine for the determination of bloodstains rarely reported. With this method we have established, the positive result of fresh bloodstains can be measured 640,000 times. Stored three years old stained blood can still be measured. Horses, cattle, sheep, dogs, pigs, chickens, ducks, pigeons, rabbits, donkeys, mules and quails were all negative. Due to the high sensitivity, specificity and easy access to reagents, this instrument does not need expensive instruments and is easy to popularize in the physical evidence test.