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大豆花叶病毒病是世界大豆产区危害较重的主要病害 ,本实验高抗SMV1株系黑农 39为母本 ,高感SMV1株系品种合丰 2 5为父本配置杂交组合 ,对F1、F2 代接种SMV1进行田间抗性鉴定和抗病性遗传学分析 ,通过接种鉴定亲本F1、F2 并代的抗性表现 ,证明 ,对SMV 1号株系的抗性是由一对显性基因决定的。利用RAPD技术在抗感池间寻找多态性标记 ,通过筛选引物 ,获得重复性最好的随机引物OPN11。并采用BSA法在黑农 39和抗病池扩增出OPN14 0 0 片段 ,在合丰 2 5和感病池扩增出OPN130 0 片段 ,在F1同时扩增出OPN14 0 0 和OPN130 0 。用该引物分析黑农 39×合丰2 5的F2 扩增个体 ,共显性的RAPD标记OPN14 0 0 / 130 0 与黑农 39抗病基因的遗传距离为 8.2cM。
Soybean mosaic virus disease is one of the most serious diseases in the soybean producing areas in the world. In this experiment, the high anti-SMV1 strain Heinong 39 was the female parent, and the highly susceptible SMV1 strain Hefeng25 was the male parent hybrid arrangement. F1 F2 generation were inoculated with SMV1 for resistance and genetic analysis of disease resistance. The resistance of F1 and F2 generations were identified by inoculation. The results showed that the resistance to SMV 1 was determined by a pair of dominant genes decided. The RAPD technique was used to find the polymorphic markers in the anti-sense pool. By selecting the primers, the random primer OPN11 with the best repeatability was obtained. BSA method was used to amplify OPN1400 fragment in Heinong 39 and disease resistant pool. OPN130 0 fragment was amplified in Hefeng 2 5 and susceptible pool, and OPN1400 and OPN130 0 were also amplified in F1. The F2 population of Heinong 39 × Hefeng 25 was analyzed by this primer. The genetic distance between the dominant dominant RAPD marker OPN14 0 0/130 0 and Heinong 39 resistant gene was 8.2 cM.