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目的观察实验小鼠脑内包囊的时空分布特点,为探讨弓形虫感染引起的情志和行为改变提供病理基础。方法弓形虫PRU株经口感染小鼠,经HE染色和免疫组化检测,在显微镜下计算前额、海马、丘脑、小脑和杏仁核部位的包囊个数,然后随机选取上述部位的5个视野拍照,计算包囊的平均密度,并用方差分析比较不同脑组织包囊密度有无统计学差异。结果弓形虫感染30和90d时,HE染色和免疫组化后显微镜观察发现,小鼠不同位置弓形虫包囊密度差异有统计学意义,其中,丘脑的包囊密度最大,其次是前额皮质、海马、杏仁核,小脑的包囊密度最小。丘脑的包囊密度显著高于其他4个脑区(P<0.01),小脑的包囊密度显著低于其它4个部位(P<0.01),而前额皮质、海马和杏仁核所含的包囊密度差异无统计学意义(P>0.05);弓形虫感染1个月时,杏仁核包囊密度明显低于感染3个月(F=18.314,P<0.001),但是小脑的包囊密度高于感染3个月(F=18.314,P<0.001)。结论弓形虫包囊在慢性感染小鼠不同脑组织内的分布具有时空特异性,这可能是其临床表现的病理学基础。
Objective To observe the spatiotemporal distribution characteristics of cysts in experimental mice and provide the pathological basis for exploring the changes of emotional and behavioral changes induced by Toxoplasma gondii infection. Methods The Toxoplasma gondii PRU strain was orally infected and the number of cysts in the forehead, hippocampus, thalamus, cerebellum and amygdala was calculated under microscope by HE staining and immunohistochemistry. Then, five fields Photographed, calculated the average density of cysts, and analysis of variance with different brain tissue cyst density whether or not statistically significant. Results Toxoplasma gondii infection at 30 and 90 days, HE staining and immunohistochemistry after microscopy showed that mice in different locations Toxoplasma cysts density was statistically significant, of which the thalamic cyst density, followed by the prefrontal cortex, hippocampus , Amygdala, cerebellar capsule density of the smallest. The cyst density of the thalamus was significantly higher than that of the other four brain regions (P <0.01), and the cyst density of the cerebellum was significantly lower than that of the other four sites (P <0.01). The cysts contained in the prefrontal cortex, hippocampus and amygdala There was no significant difference in density between the two groups (P> 0.05). At 1 month after infection with Toxoplasma gondii, the cyst density of amygdala was significantly lower than that of 3 months (F = 18.314, P <0.001) Infected for 3 months (F = 18.314, P <0.001). Conclusion The distribution of Toxoplasma gondii cysts in different brain tissues of chronically infected mice has spatio-temporal specificity, which may be the pathological basis of its clinical manifestations.