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目的探讨高压氧联合依达拉奉对脑梗死大鼠AQP9表达及脑水肿的影响。方法建立老年SD大鼠大脑中动脉闭塞(MCAQ)模型,随机分为4组:对照组、依达拉奉组、高压氧组和联合组,每组20只。对各组大鼠进行神经功能评分,检测大鼠脑组织内AQP9基因及蛋白的表达、梗死脑组织的含水量,并进行HE染色,观察病理学变化。结果各组大鼠神经功能缺损评分比较显示,联合组明显低于对照组、依达拉奉组及高压氧组;RT-PCR及Western blot检测表明,联合组AQP9的mRNA蛋白表达及梗死区脑组织含水量明显低于脑梗死组、依达拉奉组及高压氧组(P<0.05);HE染色结果显示,高压氧组、依达拉奉组较对照组炎性浸润及水肿减轻,联合组比高压氧组、依达拉奉组又有明显缓解。结论高压氧联合依达拉奉治疗可促进脑梗死大鼠神经功能的恢复。
Objective To investigate the effects of hyperbaric oxygen combined with edaravone on AQP9 expression and cerebral edema in rats with cerebral infarction. Methods The model of middle cerebral artery occlusion (MCAQ) in aged SD rats was established and randomly divided into 4 groups: control group, edaravone group, hyperbaric oxygen group and combination group, with 20 rats in each group. Neurological scores of rats in each group were measured. The expression of AQP9 gene and protein, the water content of brain tissue in infarcted rats were detected. HE staining was performed to observe the pathological changes. Results Compared with the control group, edaravone group and hyperbaric oxygen group, the neurological deficit scores of the rats in each group showed that the expression of AQP9 mRNA in the combined group was significantly lower than that in the control group The tissue water content was significantly lower than those in cerebral infarction group, edaravone group and hyperbaric oxygen group (P <0.05). HE staining showed that hyperbaric oxygen group and edaravone group had less inflammatory infiltration and edema than the control group, Group than hyperbaric oxygen group, edaravone group also significantly alleviate. Conclusion Hyperbaric oxygen combined with edaravone can promote the recovery of neurological function in rats with cerebral infarction.