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探讨细胞动力学在子宫内膜癌发病机制中的作用。方法 :对13例子宫内膜癌、癌周内膜 ,15例简单型增生过长内膜 ,13例复杂型增生过长内膜石蜡组织切片 ,作HE染色及原位末端标记 (TUNEL) ,在光镜下 ,对切片中的有丝分裂细胞及凋亡细胞进行计数。结果 :复杂型增生过长内膜有丝分裂指数 (MI)及细胞凋亡指数 (AI)均较简单型增生过长内膜显著增高 (P均 <0 0 1) ,但MI/AI比值两者无显著差异 ;子宫内膜癌灶MI、AI、MI/AI比值均较癌周内膜及复杂型增生过长内膜显著增高 (P <0 0 1,P <0 0 5,P <0 0 5)。结论 :在子宫内膜癌变的早期 ,细胞动力学遵循既定细胞数调节机制 ,即加速的细胞增殖通过加快的细胞凋亡予以平衡。当过度增殖的细胞不能通过相应增强的凋亡途径加以清除时 ,会导致DNA损伤和变异的积累 ,最终引起子宫内膜癌。
To investigate the role of cell kinetics in the pathogenesis of endometrial cancer. Methods: Thirteen cases of endometrial cancer, endometrial cancer, 15 cases of simple endometrial hyperplasia and 13 cases of complex hyperplasia of endometrial paraffin tissue sections were made for HE staining and TUNEL, Under light microscope, the mitotic cells and apoptotic cells were counted. Results: The intima mitotic index (MI) and apoptotic index (AI) in the complicated hyperplasia were significantly higher than those in the simple hyperplasia (P <0.01), but both MI / AI ratio The ratio of MI, AI and MI / AI in endometrial carcinoma was significantly higher than that in endometrial and complex endometrial hyperplasia (P <0.01, P <0.05, P <0.05) ). CONCLUSIONS: In the early stages of endometrial carcinogenesis, the cell kinetics follow a defined cell number regulation mechanism whereby accelerated cell proliferation is balanced by accelerated apoptosis. When over-proliferation of cells can not be through the corresponding enhanced apoptosis pathway to be cleared, it will lead to the accumulation of DNA damage and variation, and ultimately cause endometrial cancer.