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目的 观察幽门螺杆菌（ Ｈｐ） 培养滤液长期作用下大鼠胃粘膜组织学变化．方法 以２－５ ｇ／ Ｌ和５ ｇ／ Ｌ两种浓度的 Ｈｐ 培养滤液让大鼠自由饮用，观察不同时间大鼠胃粘膜厚度、壁细胞数、胃粘膜ＰＡＳ染色阳性层厚度，表面粘液含量的变化及肠上皮化生和异型增生的发生率．结果 实验５ ｗ ｋ 时，实验组大鼠即出现明显的胃粘膜厚度变薄，壁细胞减少，ＰＡＳ 染色阳性层变薄，表面粘液含量减少和ＤＮＡ 合成速率加快，且随着实验时间的延长，上述改变进一步加重． 至５２ ｗｋ 实验结束时，５ ｇ／ Ｌ Ｈｐ 培养滤液组大鼠腺胃粘膜肠化生，硫酸粘液阳性肠化生和异型增生的发生率（５７－１ ％ ，３９－３ ％ ，１７－８ ％ ） 显著高于２－５ ｇ／ Ｌ Ｈｐ 培养滤液组（２７－６ ％ ，１０－３ ％ ，３－４ ％ ） 和正常对照组（６－９ ％ ，０－０ ％ ，０－０ ％ ） ．结论 Ｈｐ 的毒性产物在萎缩性胃炎，肠上皮化生，异型增生乃至胃癌的发生中可能起重要作用．
Objective To observe the histological changes of gastric mucosa under the long-term effect of culture filtrate of Helicobacter pylori (Hp). Methods Rats were allowed to drink freely with filtrates of 2-5 g/L and 5 g/L Hp. The gastric mucosal thickness, number of parietal cells, gastric mucosal PAS staining positive layer thickness, and surface mucus content were observed at different times. Changes and incidence of intestinal metaplasia and dysplasia. RESULTS At the fifth week of experiment, the rats in the experimental group exhibited a marked decrease in gastric mucosal thickness, parietal cells, a thin PAS-positive staining layer, decreased surface mucus content, and increased DNA synthesis rate, which increased with the experimental time. The above changes are further aggravated. At the end of the 52 wk experiment, the intestinal metaplasia of glandular and gastric mucosa in the 5 g/L H. pylori culture filtrate group, and the incidence of positive intestinal metaplasia and dysplasia in the sulphate mucus (57-1 %, 39-3%, 17-8). %) was significantly higher than 2-5 g/L Hp culture filtrate group (27-6 %, 10-3 %, 3-4%) and normal control group (6-9 %, 0-0 %, 0-0 % ). Conclusion The toxic products of Hp may play an important role in the development of atrophic gastritis, intestinal metaplasia, dysplasia and gastric cancer.