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目的:测定平贝母药材中的主要生物碱贝母素甲和贝母素乙的含量。方法:采用高效液相色谱-蒸发光散射检测器和Agilent Hypersil BDS-C18(4.0 mm×250 mm,5μm),在流动相为乙腈-水-二乙胺(体积比70∶30∶0.03),流速0.8 mL/min,漂移管温度85℃,N2流速2.2L/min条件下测定平贝母中贝母素甲、贝母素乙的含量。结果:贝母素甲含量在3.125～100.00μg/mL、贝母素乙含量在3.14～100.05μg/mL范围内与峰面积的对数值呈线性关系,平均回收率分别为99.56%、99.33%,RSD分别为1.25%、1.53%(n=6)。结论:本方法能够测定平贝母药材中的主要生物碱贝母素甲、贝母素乙含量,可用于平贝母药材的质量控制。
OBJECTIVE: To determine the contents of major alkaloids Fritillaria and Fritillaria B in Fritillaria. Methods: High performance liquid chromatography with evaporative light scattering detector and Agilent Hypersil BDS-C18 (4.0 mm × 250 mm, 5 μm) were used in the mobile phase of acetonitrile-water-diethylamine (70:30:0.03) The flow rate of 0.8 mL / min, the drift tube temperature of 85 ° C, N2 flow rate of 2.2L / min under the conditions Fritillaria Fritillaria peach, peiminine B content. Results: The results showed that there was a linear relationship between the logarithm value of the peak area and the area of 3.15 ~ 100.00 μg / mL, and the average content of the mother of pearliserin in the range of 3.14 ~ 100.05 μg / mL. The average recoveries were 99.56% and 99.33% RSD were 1.25%, 1.53% (n = 6). Conclusion: This method can determine the main alkaloids Fritillaria cirrhosa, Fritillaria B content can be used for the quality control of Fritillaria.