一、本试验的设计思想自1978年5月中澳植物组织培养学术交流会在北京召开,英国森德兰博士介绍了液体漂浮培养花药的经验后,我所于1978年8月进行试验,证明了他的经验是可行的.这个方法保持了花药培养简单可靠的优点,而又不须要机械分裂或外科手术解剖花药,是单花粉培养一项简单的方法;更重要的是,排除了花药培养的体细胞在培养过程中的干扰.可以用少数花药获得大量愈伤组织,在育种上有现实意义. First, the design idea of ​​this experiment Since May 1978 Sino-Australian plant tissue culture academic exchange held in Beijing, the British Dr. Sunderland introduced the experience of liquid floating cultured anthers, I conducted in August 1978 test to prove His experience is feasible.This method maintains the simple and reliable advantages of anther culture without the need for mechanical splitting or surgical dissection of anthers, is a simple method of single pollen culture; more importantly, excluding anther culture Of somatic cells interfere with the process of cultivation.Many callus can be obtained with a few anthers, which has practical significance in breeding.