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本文采用LPS刺激健康人外周血的单核细胞,提取其总的RNA,采用逆转录PCR扩增目的基因,并且将获得的目的基因连接到克隆载体中,通过DNA序列分析,证实获得的目的基因是所需的IL—8的基因.
In this paper, LPS stimulation of peripheral blood mononuclear cells in healthy individuals, total RNA was extracted, the target gene was amplified by reverse transcription PCR, and the target gene was ligated into a cloning vector. Through DNA sequence analysis, it was confirmed that the obtained target gene Is the desired gene for IL-8.