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目的 建立快速、准确测定大鼠血浆中马来酸曲美布汀浓度的HPCE方法 ,并用于其在大鼠体内的药物动力学研究。方法 以盐酸麻黄碱为内标 ,0 0 3mol·L-1磷酸二氢钠 (pH 6 0 )为运行缓冲溶液 ,紫外检测波长为2 14nm。血浆样品经乙腈除蛋白后 ,于 5 0℃水浴用氮气吹干 ,残渣溶于甲醇 水 (1∶1) ,进样分析。结果 线性范围 5- 2 0 0 μg·L-1,日内RSD <14% ,日间RSD <13% ,回收率为 72 8% - 87 9% ,最低定量浓度为 5 μg·L-1。ig给药 30min后 ,血浆中药物浓度达峰值 ,T1/2 (Ke) 为 173min ,Ke为 5 6× 10 -3 min-1,AUC为 7 83μg·min·mL-1。结论 方法灵敏度高 ,操作简便 ,适用于马来酸曲美布汀的药物动力学研究
OBJECTIVE: To establish a rapid and accurate HPLC method for the determination of trimebutine maleate in rat plasma and to study its pharmacokinetics in rats. Methods Ephedrine hydrochloride was used as an internal standard, and 0 0 3 mol·L-1 sodium phosphate monobasic (pH 60) was used as the running buffer solution. The UV detection wavelength was 2 14 nm. Plasma samples were deproteinized with acetonitrile and dried in a water bath at 50 ° C with nitrogen. The residue was dissolved in methanol and water (1: 1) for injection analysis. Results The linear range was 5-20 μg · L-1, the intra-day RSD was 14%, the daytime RSD was less than 13%, the recovery was 72.8% -87.9% and the lowest concentration was 5 μg · L-1. After 30 minutes of administration, the peak plasma concentration of the drug in plasma was 173 min for T1 / 2 (Ke), 566 × 10 -3 min-1 for Ke and 7 83 μg · min · mL-1 for Ke. Conclusion The method is sensitive and easy to operate and is suitable for the pharmacokinetic study of trimebutine maleate