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25只大鼠随机分为正常对照组和灌注博莱霉素实验组。在 3d ,7d ,14d ,30d分别取肺组织 ,以免疫组织化学技术测定TGF β1蛋白表达。结果显示未经博莱霉素处理正常对照组大鼠的少数细支气管粘膜上皮细胞的胞浆有TGF β1蛋白弱表达 ;博莱霉素处理大鼠的支气管、细支气管粘膜上皮细胞则TGF β1表达更明显和范围更广 ;特别是在 3～ 7d肺泡腔和间质中出现大量的TGF β1强阳性的肺泡巨噬细胞 ,14～ 30d时在肺泡腔和间质中只有少量TGF β1阳性的肺泡巨噬细胞。提示TGF β1蛋白表达阳性的肺泡巨噬细胞在急性肺泡炎期明显增加 ,对肺纤维化的发生和发展可能起重要的作用
Twenty-five rats were randomly divided into normal control group and infusion bleomycin experimental group. Lung tissue was collected at 3d, 7d, 14d and 30d respectively, and the expression of TGFβ1 protein was detected by immunohistochemistry. The results showed that a small number of bronchial epithelial cells without bleomycin-treated normal control group of the cytoplasm of TGF β1 protein weakly expressed; bleomycin treatment of bronchial and bronchial epithelial cells TGF β1 expression Especially in a large number of alveolar macrophages with strong positive TGFβ1 in the alveolar space and interstitium from 3 to 7 days and only a few TGFβ1 positive alveoli in the alveolar space and interstitium from 14 to 30 days Macrophages. These results suggest that alveolar macrophages with positive expression of TGFβ1 may be significantly increased in acute alveolar inflammation and may play an important role in the occurrence and development of pulmonary fibrosis