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目的探讨p63基因和p73基因蛋白与毛细血管瘤发生发展的关系。方法采用免疫组化SP法检测40例儿童毛细血管瘤和20例正常人皮肤组织p63基因和p73基因蛋白的表达;对所获得的检测结果进行图像分析处理。结果在正常人皮肤组、增生期与退化期血管瘤中,p63和p73蛋白的平均吸光度分别为0.923±0.191,0.953±0.120;8.271±1.953,6.408±2.151;0.920±0.187,1.073±0.516;阳性面积率分别为0.106±0.014,0.087±0.012;0.370±0.071,0.184±0.015;0.116±0.012,0.098±0.014。增生期组与退化期组、正常人皮肤组分别比较,p63和p73阳性表达的差异均有统计学意义(P<0.05),退化期组与正常人皮肤组之间,p63阳性表达的差异无统计学意义(P>0.05)。结论p63基因在毛细血管瘤中并未作为肿瘤抑制基因起作用,相反是作为癌基因而促进内皮细胞的增殖,可能与血管形成关系密切。p73在毛细血管瘤的增生中起着重要作用。
Objective To investigate the relationship between the expression of p63 gene and p73 gene and the development of capillary hemangioma. Methods Immunohistochemical SP method was used to detect the expression of p63 gene and p73 protein in 40 children with hemangiomas and 20 normal human skin tissues. The results of the detection were analyzed by image analysis. Results In normal human skin, proliferative and degenerative hemangiomas, the average absorbance of p63 and p73 proteins were 0.923 ± 0.191 and 0.953 ± 0.120, respectively; 8.271 ± 1.953 and 6.408 ± 2.151; 0.920 ± 0.187 and 1.073 ± 0.516, respectively; Area rates were 0.106 ± 0.014,0.087 ± 0.012; 0.370 ± 0.071,0.184 ± 0.015; 0.116 ± 0.012,0.098 ± 0.014, respectively. There were significant differences in the expression of p63 and p73 between the proliferative phase group, the degenerative group and the normal human skin group (P <0.05), but there was no significant difference between the degenerative group and the normal human skin group Statistical significance (P> 0.05). Conclusion The p63 gene did not play a role as a tumor suppressor in capillary hemangiomas. On the contrary, p63 gene promotes the proliferation of endothelial cells as an oncogene, which may be closely related to the formation of blood vessels. p73 plays an important role in the proliferation of capillary hemangiomas.