论文部分内容阅读
目的探讨超抗原金黄色葡萄球菌肠毒素B亚单位(SEB)对大鼠高危角膜移植免疫排斥反应的防治作用机制。方法实验供体为30只Fisher344和4只Lewis大鼠,受体为64只Lewis大鼠。将实验受体大鼠按随机数字表法随机分为4个治疗组和1个对照组,每组12只;同时设4只Lewis大鼠行同种同体移植。治疗组术前分别用02ml不同浓度的SEB(25、50、75、100μg/kg)腹腔注射诱导免疫耐受。对照组用02ml生理盐水腹腔注射。缝线法诱导受体角膜新生血管,然后行穿透性角膜移植术,术后观察记录植片的存活状况并对受体全身免疫状态进行免疫组化染色,流式细胞分析和淋巴细胞增殖能力检测。结果对照组大鼠角膜植片的平均存活时间为(730±067)d,SEB治疗组(25、50、75、100μg/kg)植片存活时间分别为(643±127)d、(1070±250)d、(1250±141)d、(883±194)d。免疫组织化学染色和流式细胞检测显示SEB治疗组大鼠角膜植片中淋巴细胞的浸润以及外周免疫器官中淋巴细胞亚群百分数较对照组明显降低。此外,SEB治疗组中受体淋巴细胞对供体抗原刺激的反应性降低,外周血中白细胞介素(IL)2浓度降低而IL10浓度升高,其中以SEB75μg/kg组作用最明显。结论在一定剂量范围内,SEB可以诱导大鼠免疫耐受形成,减少局部和全身淋巴细胞数量,并有效防治高危角膜移植免
Objective To investigate the preventive and therapeutic mechanism of superantigen staphylococcal enterotoxin B subunit (SEB) on the rejection of high-risk corneal graft in rats. Methods Experimental donors were 30 Fisher344 and 4 Lewis rats, the recipient was 64 Lewis rats. The experimental recipient rats were randomly divided into four treatment groups and one control group according to a random number table method, with 12 rats in each group. At the same time, 4 Lewis rats were allotransplantated. In the treatment group, immunological tolerance was induced by intraperitoneal injection of different concentrations of SEB (25, 50, 75, 100μg / kg) with 02ml respectively. Control group with intraperitoneal injection of 02ml saline. The suture method was used to induce the corneal neovascularization of the recipient, then the penetrating keratoplasty was performed. The survival status of the graft was observed after operation and the immunohistochemistry, flow cytometry and lymphocyte proliferative capacity Detection. Results The mean survival time of the corneal graft in the control group was (730 ± 067) d and that in the SEB treatment group (643 ± 127) days and (1070 ± 250) d, (1250 ± 141) d, (883 ± 194) d. Immunohistochemical staining and flow cytometry showed that the infiltration of lymphocytes in corneal graft and the percentage of lymphocyte subsets in peripheral immune organs of SEB treatment group were significantly lower than those in control group. In addition, the response of donor lymphocytes to donor antigen stimulation in SEB treatment group was reduced. The concentration of interleukin (IL) 2 in peripheral blood was decreased and the concentration of IL10 was increased. The effect of SEB 75μg / kg was the most obvious. Conclusion In a certain dose range, SEB can induce the formation of immune tolerance, reduce the number of local and systemic lymphocytes, and effective prevention and treatment of high-risk corneal transplantation