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目的:检测乙型肝炎病毒相关性肾炎(HBVGN)肾组织中乙型肝炎病毒X基因(HBx)及截短型乙型肝炎病毒表面抗原(MHBst)mRNA的表达,探讨HBx、MHBst在HBVGN发生中的意义。方法:采用原位杂交的方法对40例HBVGN患者的肾组织进行HBx、MHBst mRNA检测。结果:HBVGN患者肾组织中存在HBx、MHBst mRNA,HBx、MHBst mRNA主要分布于肾小管上皮细胞的胞质内,少数肾小球上皮、内皮、系膜细胞的胞质内也有分布;肾小管上皮细胞内HBx、MHBst mRNA阳性率分别为82.5%(33/40)、80%(32/40),肾小球内HBx、MHBst mRNA阳性率分别为30%(12/40)、22.5%(9/40),HBx、MHBst mRNA在肾小管上皮细胞的表达明显高于肾小球(P<0.01)。结论:在HBVGN肾组织,特别是在肾小管上皮细胞的胞质存在HBx、MHBst mRNA的表达,提示HBV感染及其基因整合可能在HBVGN发生中有一定意义。
Objective: To detect the expression of hepatitis B virus X gene (HBx) and truncated hepatitis B virus surface antigen (MHBst) mRNA in the kidney of patients with hepatitis B virus associated nephritis (HBVGN) and to explore the role of HBx and MHBst in the pathogenesis of HBVGN Meaning. Methods: HBx and MHBst mRNA were detected by in situ hybridization in 40 patients with HBVGN. Results: HBx and MHBst mRNA were present in the kidney of patients with HBVGN. HBx and MHBst mRNA were mainly located in the cytoplasm of renal tubular epithelial cells. A small number of glomerular epithelial cells, endothelial cells and mesangial cells also distributed in the cytoplasm. The positive rates of HBx and MHBst mRNA in the glomeruli were 82.5% (33/40) and 80% (32/40), respectively. The positive rates of HBx and MHBst mRNA in glomeruli were 30% (12/40) and 22.5% / 40). The expression of HBx and MHBst mRNA in renal tubular epithelial cells was significantly higher than that in glomeruli (P <0.01). Conclusion: The expression of HBx and MHBst mRNA in the cytoplasm of HBVGN kidney tissues, especially in renal tubular epithelial cells, suggests that HBV infection and its gene integration may have some significance in the pathogenesis of HBVGN.