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目的 探讨姜黄素对实验性肾炎组织病理以及增殖细胞核抗原 (PCNA)的影响。方法雄性SD大鼠 72只随机分成 3组 ,正常对照组 :注射生理盐水 ;模型组 :尾静脉注射兔抗鼠肾毒血清0 5ml/d ,连用 2d ,腹腔注射二甲亚砜 0 5ml·kg 1·d 1;姜黄素组 :尾静脉注射兔抗鼠肾毒血清 0 5ml/d ,连用d ,同时腹腔注射姜黄素 5 0mg·kg 1·d 1。分别于第 3、7、14、2 8d处死大鼠 ,取肾组织分送常规病理、免疫组化及透射电子显微镜检查。结果 姜黄素组肾小球细胞数明显少于模型组 ;间质炎细胞浸润亦较模型组明显减少 ;14d后小管间质损伤指数也低于模型组。电镜显示 ,姜黄素可明显阻止肾小球上皮细胞足突融合以及基膜增厚 ,抑制系膜细胞、内皮细胞增殖 ,减少肾组织内炎细胞浸润 ,尤其是对间质单核样细胞浸润的减少作用更为明显 ;免疫组化也证实姜黄素应用 7d后即可降低PCNA表达。结论 姜黄素能明显改善肾炎时肾小球超微结构的改变 ,并抑制系膜细胞、内皮细胞增殖 ,减轻小管间质损伤及肾组织内炎性细胞的浸润。
Objective To investigate the effects of curcumin on histopathology and proliferating cell nuclear antigen (PCNA) in experimental nephritis. Methods Seventy-two male Sprague-Dawley rats were randomly divided into 3 groups. The normal control group was injected with normal saline. The model group was injected with rabbit anti-rat nephrotoxic serum (0 ml/d) for 2 days. Dimethyl sulfoxide was injected intraperitoneally. 1 · d 1; curcumin group: tail vein injection of rabbit anti-rat nephrotoxic serum 0 5ml / d, with d, while intraperitoneal injection of curcumin 50mg · kg 1 · d 1. The rats were sacrificed on the 3rd, 7th, 14th, and 28th day respectively. The kidney tissues were collected and sent for routine pathology, immunohistochemistry and transmission electron microscopy. Results The number of glomerular cells in the curcumin group was significantly lower than that in the model group; the infiltration of the interstitial cells was also significantly reduced compared with the model group; the tubulointerstitial damage index was also lower than the model group after 14 days. Electron microscopy showed that curcumin can significantly prevent the glomerular epithelial cell foot fusion and basement membrane thickening, inhibit the proliferation of mesangial cells, endothelial cells, and reduce the infiltration of inflammatory cells in the renal tissue, especially for interstitial mononuclear cell infiltration The reduction effect was more pronounced; immunohistochemistry also confirmed that curcumin could reduce PCNA expression after 7 days of application. Conclusion Curcumin can significantly improve glomerular ultrastructural changes in nephritis, and inhibit the proliferation of mesangial cells and endothelial cells, and reduce tubulointerstitial injury and inflammatory cell infiltration in renal tissue.