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目的从细胞膜流动性和胞内遗传物质损伤程度对纳米氧化铁的氧化作用进行检测与评价。方法以1,6-二苯基-1,3,5-己三烯(DPH)作为荧光标记,通过荧光分光光度计检测荧光强度变化,计算细胞膜的流动度;单细胞凝胶电泳(SCGE)检测胞内遗传物质在DNA水平的氧化损伤情况。结果仅在128μg/ml剂量水平观察到细胞膜流动性的降低,但8,32,128μg/ml 3个剂量组均未引起细胞内DNA损伤。结论一定剂量的纳米氧化铁(Fe2O3)颗粒可引起细胞膜结构的氧化损伤,表现为膜流动性的降低。但各剂量组均未观察到纳米Fe2O3的遗传毒性效应。
OBJECTIVE To detect and evaluate the oxidation of nano-iron oxide from the membrane fluidity and the degree of intracellular genetic material damage. Methods Fluorescence intensity was measured by fluorescence spectrophotometer with 1,6-diphenyl-1,3,5-hexatriene (DPH) as fluorescent label, and the cell membrane fluidity was calculated. Single cell gel electrophoresis (SCGE) Detection of intracellular genetic material at the DNA level of the oxidative damage. As a result, cell membrane fluidity reduction was observed only at a dose level of 128 μg / ml, but none of the 8, 32, 128 μg / ml 3 dose groups caused DNA damage in cells. Conclusion A certain dose of Fe2O3 particles can cause oxidative damage of cell membrane structure, showing the decrease of membrane fluidity. However, the genotoxic effects of Fe2O3 nanoparticles were not observed in all dose groups.