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目的对医院细菌室送检粪便标本中分离出的艰难梭菌,进行毒素基因以及耐药基因的初步分析。方法将细菌室收集到的112份粪便标本,经选择性厌氧培养,谷氨酸脱氢酶(GDH)以及API 20A生化条鉴定后,分离出12株艰难梭菌,分别采用PCR的方法检测tcdA和tcdB基因、二元毒素基因,克林霉素抗性基因(ermB)。结果12株艰难梭菌中8株为毒素基因阳性,其中tcdA+tcdB+为5株,占62.5%;tcdA-tcdB+为3株,占37.5%;二元毒素基因均为阴性;耐药基因ermB阳性为4株,占50%。结论艰难梭菌tcdA-tcdB+毒株所占比例增加,临床单独检测A毒素易造成漏检;艰难梭菌耐药现象值得关注。
Objective To analyze the toxin genes and drug resistance genes isolated from C. difficile isolated from the stool samples in the hospital bacterial room. Methods Stool samples were collected from 112 bacterial strains. After selective anaerobic culture, glutamate dehydrogenase (GDH) and API 20A biochemical identification, 12 strains of C. difficile were isolated and detected by PCR tcdA and tcdB genes, binary toxin genes, clindamycin resistance genes (ermB). Results Totally 8 strains of C. difficile were positive for toxins, including 5 strains of tcdA + tcdB + (62.5%), 3 strains of tcdA-tcdB + (37.5%), 2 toxin genes 4 strains, accounting for 50%. Conclusion Clostridium difficile tcdA-tcdB + increased the proportion of strains, A toxin alone clinically detected easily lead to undetected; C. difficile drug resistance is worth attention.