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目的 :探讨新生期母婴分离(maternal separation,MS)作为一种生命早期应激对成年小鼠肿瘤生长的影响。方法 :C57BL/6小鼠在出生后3周内,每天和母鼠分开饲养6 h,建立小鼠MS模型。待小鼠6周龄(出生后第43天)时,用高架十字迷宫(elevated plus maze,EPM)行为学实验检测MS对小鼠焦虑水平的影响,并于出生后第45天时皮下接种胰腺癌Panc-02细胞,建立小鼠胰腺癌移植瘤模型,观察小鼠胰腺癌移植瘤的生长状况。结果 :新生期MS能明显降低雄性小鼠成年后在EPM开放臂中的活动时间(P<0.01)和活动路程百分比(P<0.05),并延长其进入开放臂的等待时间(P<0.01),提示MS提高了雄性小鼠成年后的焦虑水平。同时,MS能明显促进胰腺癌Panc-02细胞皮下移植瘤的生长(P<0.01)。胰腺癌Panc-02细胞接种小鼠35 d后,雄性MS组小鼠的移植瘤质量较正常对照组小鼠增加了56.8%,差异有明显的统计学意义(P<0.05)。然而,MS对雌性小鼠成年后的焦虑水平及胰腺癌移植瘤的生长均无明显影响(P值均>0.05)。结论 :生命早期母婴分离具有促进成年小鼠肿瘤生长的作用,且该作用可能存在性别差异。
Objective: To investigate the effect of maternal separation (MS) as a kind of early life stress on tumor growth in adult mice. Methods: C57BL / 6 mice were bred separately from their mother rats for 6 h every 3 weeks after birth. MS models of mice were established. When mice were 6 weeks old (43 days after birth), the effect of MS on the anxiety level of mice was examined by an elevated plus maze (EPM) behavioral test and pancreatic cancer was inoculated subcutaneously on the 45th day after birth Panc-02 cells to establish a mouse pancreatic cancer xenograft model to observe the growth of mouse pancreatic cancer xenografts. Results: Neonatal MS significantly decreased the activity time (P <0.01) and the percentage of active path length (P <0.05) in adult open arms and prolonged the waiting time (P <0.01) , Suggesting that MS increased the level of anxiety in the adult male mice. Meanwhile, MS could significantly promote the growth of pancreatic cancer Panc-02 cells subcutaneously (P <0.01). After being inoculated with pancreatic cancer Panc-02 cells for 35 days, the mass of transplanted tumors in male MS group increased by 56.8% compared with that in normal control group (P <0.05). However, MS had no significant effect on the adult anxiety level and the growth of pancreatic cancer (all P> 0.05). Conclusion: The separation of mother and infant in early life has the effect of promoting tumor growth in adult mice, and the effect may have gender differences.