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目的探讨C基因截短型HBV突变体对HBV复制的影响。方法构建C基因截短的HBV表达载体pHBV-ΔC,瞬时转染HepG2细胞,聚丙烯酰胺凝胶电泳(SDS-PAGE)Western blot检测S蛋白的表达;酶联免疫吸附实验(ELISA)定量分析S蛋白的表达量。pHBV-ΔC与adwR9共转染HepG2细胞,以pcDNA3与adwR9共转染为对照,荧光定量PCR检测培养上清液及胞内病毒量。结果HBV C基因截短对S蛋白的表达量没有影响,pHBV-ΔC与adwR9共转染组上清液和细胞内病毒量较对照组降低。结论C基因截短型HBV突变体可导致HBV复制下降。
Objective To investigate the effect of truncated HBV C mutants on HBV replication. Methods The recombinant plasmid pcDNA3.1 (+) was transfected into HepG2 cells, and the expression of S protein was detected by SDS-PAGE and Western blot. The results of enzyme-linked immunosorbent assay (ELISA) The amount of protein expression. HepG2 cells were co-transfected with pHBV-ΔC and adwR9, cotransfected with pcDNA3 and adwR9 as controls, and the supernatant and intracellular viral load were detected by fluorescence quantitative PCR. Results The truncation of HBV C gene had no effect on the expression of S protein. The amount of virus in supernatant and intracellular of co-transfection group of pHBV-ΔC and adwR9 was lower than that of control group. Conclusion C gene truncated HBV mutants can lead to HBV replication decreased.