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目的 探讨肿瘤坏死因子 (TNF)相关的凋亡诱导配体基因 (TRAIL)用于人大肠癌细胞株HT2 9基因治疗的实验研究。方法 将重组腺病毒载体 (Ad)介导的TRAIL基因作用于人大肠癌细胞株HT2 9,通过相差显微镜、MTT比色法和流式细胞仪 ,研究分析其对HT2 9细胞作用的效果。结果Ad/GT TRAIL能引起HT2 9细胞出现明显的形态学改变 ,对HT2 9细胞的生长抑制率和凋亡诱导率分别为 5 4 .3%和 11.1% ;联合Ad/PGK GV16后 ,生长抑制率和凋亡诱导率均显著提高 (P <0 .0 5 ) ,分别为 82 .7%和 2 4 .6 %。结论 Ad/GT TRAIL能有效诱导HT2 9的凋亡从而抑制HT2 9的生长 ,联合Ad/PGK GV16后将显著提高其疗效。
Objective To investigate the effect of tumor necrosis factor (TNF)-related apoptosis-inducing ligand gene (TRAIL) on gene therapy of human colorectal cancer cell line HT29. Methods The recombinant adenovirus vector (Ad)-mediated TRAIL gene was applied to human colorectal cancer cell line HT2 9, and the effect of TRAIL gene on HT29 cells was analyzed by phase contrast microscopy, MTT colorimetry and flow cytometry. Results Ad/GT TRAIL could induce obvious morphological changes in HT2 9 cells. The growth inhibitory rate and apoptosis inducing rate of HT2 9 cells were 54.3% and 11.1%, respectively. After combined with Ad/PGK GV16, growth inhibition was observed. The rate and induction rate of apoptosis were significantly increased (P < 0.05), which were 82.7% and 24.6%, respectively. Conclusion Ad/GT TRAIL can effectively induce the apoptosis of HT2 9 and inhibit the growth of HT29. Combined with Ad/PGK GV16, it will significantly improve the efficacy.