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目的研究过氧化酶增殖因子活化受体γ(peroxisome proliferator-activated receptor gamma,PPARγ)和血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)在胃癌组织中表达的相关性,分析其表达与临床病理因素之间的关系。方法 2005年3月-2009年3月间采用逆转录-聚合酶链反应方法检测36例胃癌手术标本中PPARγ和VEGF-C mRNA的表达,同时选取相同患者的胃正常组织作为对照。结果 PPARγmRNA在胃癌中的表达量高于癌旁正常组织,两者的差异有统计学意义(P=0.007);VEGF-C在胃癌中的表达量高于癌旁正常组织,两者的差异有统计学意义(P=0.004);PPARγ的表达与VEGF-C表达无关联性(r=0.135,P=0.414);PPARγ表达与胃癌组织中浸润程度有关(χ2=4.620,P=0.032)、淋巴结转移有关(χ2=15.753,P=0.000)和临床病理分期有关(χ2=4.610,P=0.032);VEGF-C表达与胃癌组织中淋巴结转移有关(χ2=4.729,P=0.030)、远处转移有关(χ2=4.064,P=0.044)和临床病理分期有关(χ2=6.300,P=0.012)。结论 PPARγ和VEGF-C可能在胃癌新生淋巴管形成中起重要作用,两者的表达水平与胃癌患者的病情判断及预后评价密切相关。
Objective To investigate the expression of peroxisome proliferator-activated receptor gamma (PPARγ) and vascular endothelial growth factor C (VEGF-C) in gastric carcinoma and to analyze their expression And the relationship between clinicopathological factors. Methods The expression of PPARγ and VEGF-C mRNA in 36 specimens of gastric cancer from March 2005 to March 2009 were detected by reverse transcription polymerase chain reaction (RT-PCR). Meanwhile, normal gastric tissues of the same patients were selected as control. Results The expression of PPARγmRNA in gastric cancer was higher than that in adjacent normal tissues, the difference was statistically significant (P = 0.007). The expression of VEGF-C in gastric cancer was higher than that in adjacent normal tissues (P = 0.004). There was no correlation between PPARγ expression and VEGF-C expression (r = 0.135, P = 0.414). The expression of PPARγ was correlated with the degree of infiltration in gastric cancer tissues (χ2 = 4.620, (Χ2 = 4.753, P = 0.000) and clinicopathological stage (χ2 = 4.610, P = 0.032). The expression of VEGF-C was correlated with lymph node metastasis in gastric cancer tissues (χ2 = 4.729, P = 0.030) (Χ2 = 4.064, P = 0.044) and clinicopathological stage (χ2 = 6.300, P = 0.012). Conclusion PPARγ and VEGF-C may play an important role in the formation of lymphangiogenesis in gastric cancer. The expression of PPARγ and VEGF-C are closely related to the prognosis and prognosis of gastric cancer.