童哲等发现光可促进白芥愈伤组织的生长和器官分化。在细胞水平上研究发现红光能引起黄化小麦、燕麦叶肉原生质体膨大。王小菁等发现红光抑制双子叶植物绿豆下胚轴切段伸长,但能引起黄化绿豆幼苗下胚轴原生质体的膨大。我们以绿豆幼苗下胚轴原生质体为材料,研究红、白、蓝光在细胞壁再生和细胞分裂中的作用。 1 材料与方法 1.1 光源装置 以20W×3荧光灯为光源,经红、蓝色滤光片(德国罗姆和哈斯RoHM &HAAS公司生产)而获得红光和蓝光,光辐照度为2W/m~2,光谱见童哲(植物生理学通讯,1989(2):28～31)。白光以高压碘钨灯为光源,光强为40W/m~2。 1.2 材料培养 绿豆(Phaseolus radiatus)种子经75％乙醇和0.1％升汞消毒,无菌水冲洗3遍,播于铺有滤纸的三角瓶中,保持合适水分,22±1℃培养箱中培养48h。 1.3 原生质体制备 选下胚轴长2～2.5cm的绿豆幼苗,从弯钩下3mm处,向下切取 Tetsu et al. Found that light can promote white mustard callus growth and organ differentiation. Research at the cellular level found that red light can cause yellowing of wheat, oat mesophyll protoplast swelling. Wang Xiaojing and other found that red light inhibited dicotyledon mung bean hypocotyl cut segment elongation, but can cause hypocotyl prothallium green bean seedling enlargement. We hypocotyl protoplasts from mung bean seedlings as materials, the red, white and blue light in the cell wall regeneration and cell division in the role. 1 Materials and methods 1.1 light source device 20W × 3 fluorescent lamp as a light source, the red and blue filters (Germany Rohm and Haas RoHM & HAAS company) to obtain red and blue light, light irradiance of 2W / m ~ 2, spectrum see Tong Zhe (Phytophysiology Newsletter, 1989 (2): 28 ~ 31). White light to high-pressure iodine tungsten light source, light intensity 40W / m ~ 2. 1.2 Materials Cultivation Phaseolus radiatus seeds were sterilized with 75% ethanol and 0.1% mercuric chloride, washed three times with sterile water and sowed in filter paper-filled triangular flask to maintain proper moisture. The seeds were incubated in a 22 ± 1 ℃ incubator for 48h . 1.3 protoplast selection of hypocotyls long mung bean seedlings 2 ~ 2.5cm, 3mm from below the hook, cut down