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[目的]为了明确壳聚糖对番番茄茄幼苗抗灰霉病的诱导作用。[方法]采用叶面喷施的方法,测定了壳聚糖对番茄灰霉病诱抗效果以及番茄叶片中叶绿素含量、可溶性蛋白质、可溶性糖、愈创木酚过氧化物酶(POD)、丙二醛(MDA)、脯氨酸的影响。[结果]壳聚糖对番茄灰霉病的诱抗效果14d时达到58.26%;与灰霉病菌处理组相比,壳聚糖处理组番茄幼苗叶片中叶绿素含量最高增加34.63%、可溶性蛋白含量增加5.30%、可溶性糖含量增加10.83%、游离脯氨酸含量增加16.21%、POD含量增加16.88%、MDA含量降低16.54%。[结论]外源壳聚糖可以提高番茄幼苗光合利用率和保护酶活性从而提高番茄幼苗对灰霉病的抗性。
[Objective] The research aimed to clarify the effect of chitosan on the induction of resistance to Botrytis cinerea in tomato seedlings. [Method] The inducing effect of chitosan on tomato gray mold and the content of chlorophyll, soluble protein, soluble sugar, guaiacol peroxidase (POD), soluble sugar, Dialdehyde (MDA), proline. [Result] The inductive effect of chitosan on tomato gray mold was 58.26% on the 14th day. Compared with the Botrytis cinerea group, the chlorophyll content in tomato seedling leaves increased by 34.63% and the content of soluble protein increased 5.30%, soluble sugar content increased 10.83%, free proline content increased 16.21%, POD content increased 16.88%, MDA content decreased 16.54%. [Conclusion] Exogenous chitosan can improve the photosynthetic utilization rate and the activity of protective enzymes in tomato seedlings and thus increase the resistance of tomato seedlings to Botrytis cinerea.