化学物质对不同发育天数海湾扇贝幼虫变态的诱导

来源 :中国水产科学 | 被引量 : 0次 | 上传用户:hushengming1
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
自海湾扇贝(Argopectenirradians)受精后第10天起,定期取一定量海湾扇贝幼虫,分别置于不同浓度梯度的KCl、肾上腺素、去甲肾上腺素、L-DOPA、5-羟色胺、GABA、茶碱和咖啡因等8种诱导物中,处理时间为8h。实验后第14天取出幼虫观察显示,这8种诱导物对不同发育天数海湾扇贝幼虫的变态有着不同的诱导作用。13.42×10-3和20.13×10-3mol/L的KCl对第12天幼虫的变态有抑制作用,变态提高率为负值;当幼虫发育至第13和14天时,上述两浓度的KCl能够明显诱导幼虫变态,变态率均提高20%以上,而对于第16天的幼虫诱导作用有所减弱,变态提高率有所降低;26.85×10-3mol/L的KCl对第12和13天幼虫的变态均有抑制作用,变态提高率为负值,对第14和16天幼虫的变态却有明显持续的诱导作用,变态率分别提高22.98%和37.5%。神经递质肾上腺素、去甲肾上腺素、L-DOPA、5-羟色胺和GABA的诱导作用规律基本相似,即对第13天海湾扇贝幼虫的变态有明显的抑制作用,变态提高率均为负值,而对第14天幼虫的诱导作用较显著。茶碱和咖啡因作为影响细胞内cAMP的物质,其诱导作用规律与神经递质有所不同,对第13天海湾扇贝幼虫变态的诱导效果最好。 From the tenth day after fertilization, Argopecten irradians regularly took a certain amount of Gulf scallop larvae, and placed them in different concentrations of KCl, epinephrine, norepinephrine, L-DOPA, serotonin, And caffeine and other 8 kinds of inducer, the treatment time is 8h. The observation of larvae removed on the 14th day after the experiment showed that the eight inductors had different induction effects on the metamorphosis of Gulf scallop larvae on different days of development. The KCl of 13.42 × 10-3 and 20.13 × 10-3mol / L inhibited the metamorphosis of the 12th day larvae, and the rate of improvement of metamorphosis was negative. When the larvae developed to the 13th and 14th days, the above two concentrations of KCl could be obviously Induced larval metamorphosis and metamorphosis rates were increased by more than 20%, while on the 16th day larval induction was weakened, the rate of metamorphosis decreased; 26.85 × 10-3mol / L of KCl on the 12th and 13th day larva metamorphosis All had inhibitory effect. The rate of improvement of metamorphosis was negative, and the metamorphosis of larvae on the 14th and 16th days was significantly and continuously induced. The metamorphosis rate increased by 22.98% and 37.5% respectively. The regulation of neurotransmitter adrenergic, norepinephrine, L-DOPA, serotonin and GABA was similar, that is to say, the metamorphosis of bay scallop larvae on day 13 was significantly inhibited and the rate of abnormal metamorphosis was negative , While on the 14th day the induction of larvae more significant. Theophylline and caffeine, as the substances that affect intracellular cAMP, had different inducing effects on neurotransmitters and had the best induction effect on metamorphosis of Gulf scallop larvae on the 13th day.
其他文献
目的总结桡骨远端骨巨细胞瘤瘤段切除后,采用自体腓骨近段移植行腕关节部分融合术(腓骨-舟月骨融合)的临床效果。方法 2008年5月至2010年1月,收治7例桡骨远端Ⅱ、Ⅲ级(Campan
研究比较了汕优63和金优77两种不同基因型水稻在幼苗期于武昌东湖水中生长时体内磷含量及其变化速率.结果表明,金优77较汕优63能更有效地利用天然水中的磷.将湖水中的磷划分
对烤烟品种G80的光合特性进行了系统研究。结果表明 ,G80叶片光饱和点、净光合速率、光补偿点、表观量子效率、CO2 饱和点、暗呼吸速率均随着叶片的生长而增大 ,在旺长期达到
本文报道了养殖条件下暗纹东方鲀的摄食行为和不同生长阶段食物组成的变化,肠道食物分析表明,除主食配合饲料外,同时也摄食一些天然饵料(底栖类及小杂鱼等)。其肠长与体长呈
采用田间试验研究了灌水和过量施钾对烤烟 (NicotianatabacumL )养分含量和产量品质的影响 .结果表明 ,在烤烟N∶K2 O用量比达到 1∶3的基础上随钾用量增加 ,烟株体内营养元
从众多水稻品种及单植株中评价筛选少数具有化感特性的材料,是选育水稻化感新品种取得突破的关键.具有化感特性的水稻品种及单植株通过产生和释放特定次生物质到环境中而显示化
根据四川井研县坡耕地实验点对不同坡度、不同耕作方式情况下水土流失的实测资料 ,引入神经网络建模方法 ,建立了不同坡度下顺坡垄作和横坡垄作的次降雨产沙网络模型 ,得出前
利用阴离子交换柱Q Hyper D对双峰驼精清进行分离,大鼠垂体组织培养及母驼肌注活性组分,RIA法测定垂体组织培养液和母驼血浆中LH及FSH的浓度.实验结果表明,驼精清经Q Hyper D
研究了微囊藻毒素(MC-LR)对咸淡水褶皱臂尾轮虫的急性毒性效应.采用克隆培养法,通过编制生命表计算内禀增长率(rm)、周限增长率(l)、净生殖率(R0)和世代时间(T),研究MC-LR的浓度在1~20mg/mL之间对轮虫种群增长参数的影响.结果表明,MC-LR的24h LC50为124.87mg/mL;各实验组轮虫的存活率和繁殖率及种群增长参数均低于对照组,其中以R0的改变最明显,对照组是实验组
研究了火棘果实发育成熟和贮藏过程中呼吸作用、相关氧化酶、营养成分的动态变化以及植物生长调节剂赤霉素(GA_3)和6-苄基腺嘌呤(6-BA)的作用,果实采收前2个月用80mg/kgGA_3