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自海湾扇贝(Argopectenirradians)受精后第10天起,定期取一定量海湾扇贝幼虫,分别置于不同浓度梯度的KCl、肾上腺素、去甲肾上腺素、L-DOPA、5-羟色胺、GABA、茶碱和咖啡因等8种诱导物中,处理时间为8h。实验后第14天取出幼虫观察显示,这8种诱导物对不同发育天数海湾扇贝幼虫的变态有着不同的诱导作用。13.42×10-3和20.13×10-3mol/L的KCl对第12天幼虫的变态有抑制作用,变态提高率为负值;当幼虫发育至第13和14天时,上述两浓度的KCl能够明显诱导幼虫变态,变态率均提高20%以上,而对于第16天的幼虫诱导作用有所减弱,变态提高率有所降低;26.85×10-3mol/L的KCl对第12和13天幼虫的变态均有抑制作用,变态提高率为负值,对第14和16天幼虫的变态却有明显持续的诱导作用,变态率分别提高22.98%和37.5%。神经递质肾上腺素、去甲肾上腺素、L-DOPA、5-羟色胺和GABA的诱导作用规律基本相似,即对第13天海湾扇贝幼虫的变态有明显的抑制作用,变态提高率均为负值,而对第14天幼虫的诱导作用较显著。茶碱和咖啡因作为影响细胞内cAMP的物质,其诱导作用规律与神经递质有所不同,对第13天海湾扇贝幼虫变态的诱导效果最好。
From the tenth day after fertilization, Argopecten irradians regularly took a certain amount of Gulf scallop larvae, and placed them in different concentrations of KCl, epinephrine, norepinephrine, L-DOPA, serotonin, And caffeine and other 8 kinds of inducer, the treatment time is 8h. The observation of larvae removed on the 14th day after the experiment showed that the eight inductors had different induction effects on the metamorphosis of Gulf scallop larvae on different days of development. The KCl of 13.42 × 10-3 and 20.13 × 10-3mol / L inhibited the metamorphosis of the 12th day larvae, and the rate of improvement of metamorphosis was negative. When the larvae developed to the 13th and 14th days, the above two concentrations of KCl could be obviously Induced larval metamorphosis and metamorphosis rates were increased by more than 20%, while on the 16th day larval induction was weakened, the rate of metamorphosis decreased; 26.85 × 10-3mol / L of KCl on the 12th and 13th day larva metamorphosis All had inhibitory effect. The rate of improvement of metamorphosis was negative, and the metamorphosis of larvae on the 14th and 16th days was significantly and continuously induced. The metamorphosis rate increased by 22.98% and 37.5% respectively. The regulation of neurotransmitter adrenergic, norepinephrine, L-DOPA, serotonin and GABA was similar, that is to say, the metamorphosis of bay scallop larvae on day 13 was significantly inhibited and the rate of abnormal metamorphosis was negative , While on the 14th day the induction of larvae more significant. Theophylline and caffeine, as the substances that affect intracellular cAMP, had different inducing effects on neurotransmitters and had the best induction effect on metamorphosis of Gulf scallop larvae on the 13th day.