论文部分内容阅读
以羊草(Leymus chinensis)-内生真菌共生体为研究对象,分别在野外样地和室内盆栽两种实验条件下研究了内生真菌感染对土壤特性和微生物群落结构的影响。结果显示:在处理时间较长并伴随有枯落物分解的羊草样地中,内生真菌感染促进了土壤氮(N)的积累,提高了30天培养时间内土壤初始碳(C)矿化速率和前3天土壤矿化量和土壤矿化总量;而在处理时间较短且没有地上枯落物分解的盆栽羊草中,内生真菌感染对土壤的C、N含量及C矿化均无显著影响。无论是野外样地还是室内盆栽实验,内生真菌感染均未引起土壤微生物磷脂脂肪酸种类的变化,但内生真菌感染均有提高土壤微生物生物量的趋势,内生真菌显著增加了盆栽羊草土壤中细菌、革兰氏阴性细菌、真菌磷脂脂肪酸含量和磷脂脂肪酸总量,增加了羊草样地土壤中革兰氏阳性细菌和放线菌的磷脂脂肪酸含量。总体看来,内生真菌感染能够改变土壤N积累和C矿化率,并且改变土壤中微生物群落的结构,这有助于进一步认识内生真菌与羊草之间的共生关系及其在生态系统C、N循环中所起的作用。
Taking Leymus chinensis-endophytic fungi symbiosis as the research object, the effects of endophyte infection on soil characteristics and microbial community structure were studied in field and potted plants respectively. The results showed that endophytic fungal infection promoted the accumulation of soil N and increased the initial carbon (C) content in the soil during the 30-day incubation period in the leymus chinensis with longer treatment time and litter decomposition. The rate of soil mineralization and the amount of soil mineralization in the first three days. In the potted Leymus chinensis, which had a shorter treatment time and no litter on the ground, the contents of C and N in the soil and C No significant effect. Endophytic fungi infection did not change the types of soil microbial phospholipid fatty acids in both field plots and in-house pot experiments, but both endophytic fungi infection increased soil microbial biomass. Endophytic fungi significantly increased potted soil Bacteria, Gram-negative bacteria, fungal phospholipid fatty acid content and total phospholipid fatty acids increased the phospholipid fatty acid content of Gram-positive bacteria and actinomycetes in the soil of Leymus chinensis. Overall, endophytic fungal infection can change soil N accumulation and C mineralization rate, and change the structure of soil microbial community, which helps to further understand the symbiotic relationship between endophytic fungi and Leymus chinensis and its ecological system C, N cycle of the role played.