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低能离子注入可以引起生物DNA分子结构的变异,但用蛋白质组学方法对低能离子注入后所引起的蛋白质诱变效应的研究尚缺乏文献报道。以向日葵种子为实验材料,对N+注入后种子的蛋白质组进行了初步分析, 共获得369个蛋白质点,其中包括对照种子中的8个特异点和处理种子中的4个特异点。这些特异点的分子量在15～34 ku之间。通过基质辅助激光解吸电离飞行时间质谱鉴定发现对照种子蛋白质中的No.29特异蛋白与MADS盒转录因子HAM59有23.48%的匹配率。处理种子中的No.279特异蛋白与亮氨酸拉链蛋白的同源蛋白HAHB-4有23.20%的匹配率。
Low-energy ion implantation can cause the variation of the molecular structure of biological DNA, but the research on the protein mutagenesis induced by low-energy ion implantation using proteomics has not been reported in the literature. Taking sunflower seeds as experimental material, the proteome of seeds after N + injection was preliminarily analyzed. A total of 369 protein spots were obtained, including 8 specific spots in the control seed and 4 specific spots in the treated seed. The molecular weight of these specific points is between 15 and 34 ku. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identified a 23.48% match between the No. 29 specific protein in the control seed protein and the MADS box transcription factor HAM59. The No.279-specific protein in seeds was matched with the homologous protein HAHB-4 of leucine zipper protein by 23.20%.