目的:建立HPLC测定清热卡森颗粒中秦皮乙素含量的方法。方法:反相高效液相色谱法。色谱柱为Kromasil C18(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(10∶90)流动相,流速为1.0 mL·min-1,柱温为40℃,检测波长为346 nm,进样量10μL。结果:秦皮乙素的线性范围为0.010 1～0.253 2μg(r=0.999 9)。平均加样回收率99.1%,RSD 1.14%。结论:方法操作简单,灵敏,准确,重复性好,可用于清热卡森颗粒的质量控制。 Objective: To establish a HPLC method for the determination of aesculin in Qingre Carson granules. Methods: Reversed-phase high performance liquid chromatography. The column was Kromasil C18 (4.6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile-0.1% phosphoric acid (10:90) at a flow rate of 1.0 mL · min-1. The column temperature was 40 ℃ and the detection wavelength was 346 nm. Injection volume 10μL. Results: The linear range of aesculetin was 0.010 1 ~ 0.253 2 μg (r = 0.999 9). The average recovery rate of 99.1%, RSD 1.14%. Conclusion: The method is simple, sensitive, accurate and reproducible. It can be used for the quality control of heat-cured Carsen particles.