目的:检测宫颈活检组织中上皮内瘤变(CIN)及其旁组织中hTERC基因的表达情况,对比分析其阳性率的变化,试图找到基因水平上CIN手术治疗范围。方法:采用荧光原位杂交技术(FISH)检测63例宫颈活检标本中hTERC基因的表达变化情况。结果:宫颈高级别上皮内瘤变(HSIL)区、瘤变边缘≤1 mm、1 mm<瘤变边缘≤2 mm、2 mm<瘤变边缘≤3 mm以及3 mm<瘤变边缘≤4 mm的hTERC基因阳性率分别为100%、92.31%、87.18%、64.10%和30.77%,其中,HSIL与瘤变边缘2 mm以内范围各点比较差异无统计学意义(P>0.05);HSIL与瘤变边缘2 mm以外范围各点比较差异有统计学意义(P<0.05);宫颈低级别上皮内瘤变(LSIL)区、瘤变边缘≤1 mm范围、1 mm<瘤变边缘≤2 mm、2 mm<瘤变边缘≤3 mm以及3 mm<瘤变边缘≤4 mm的hTERC基因阳性率分别为41.67%、25.00%、4.17%、4.17%和4.17%,其中,LSIL与瘤变边缘1 mm以内范围各点比较差异无统计学意义(P>0.05),而LSIL与瘤变边缘1 mm以外范围点比较差异有统计学意义(P<0.05)。结论:活检标本中,随着距瘤变区范围的增加,hTERC基因的阳性率明显下降,其中,距瘤变边缘>2 mm和>1 mm或许可以分别作为HSIL和LSIL基因水平上(更精确意义上的)CIN的手术治疗范围。 OBJECTIVE: To detect the expression of hTERC gene in cervical intraepithelial neoplasia (CIN) and its adjacent tissues, and to compare the positive rate of hTERC gene mutation in order to find out the extent of CIN surgery on the gene level. Methods: Fluorescence in situ hybridization (FISH) was used to detect the expression of hTERC gene in 63 cervical biopsies. Results: In the high grade intraepithelial neoplasia of the cervix (HSIL), the edge of tumor was less than or equal to 1 mm, the diameter of tumor was less than or equal to 2 mm, the diameter of tumor was less than or equal to 3 mm and the edge of tumor was less than or equal to 4 mm The positive rate of hTERC gene was 100%, 92.31%, 87.18%, 64.10% and 30.77%, respectively. There was no significant difference between HSIL and each point within 2 mm (P> 0.05) (P <0.05); low grade intraepithelial neoplasia (LSIL), the edge of tumor less than 1 mm range, 1 mm 0.05). However, there was significant difference between LSIL and 1 mm beyond the edge of tumor (P <0.05). CONCLUSIONS: The positive rate of hTERC gene in the biopsy specimens decreased significantly with the distance from the neoplastic region, of which,> 2 mm and> 1 mm from the edge of the neoplasm may be used as the HSIL and LSIL gene levels (more precisely In the sense of) CIN surgical treatment range.