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目的观察不同剂量异丙酚对大鼠缺血再灌注肝脏的抗氧化作用。方法60只Wistar 大鼠随机分为3组,每组20只,A组:对照组;B组:异丙酚2 mg·h-1·100 g-1;C组:异丙酚4 mg·h-1·100 g-1,每组又分为4个亚组,每亚组5只,分别为仅麻醉后开腹(a亚组);缺血30 min再灌注30 min (b亚组);缺血60min再灌注60min(c亚组);缺血60min再灌注120min(d亚组)。B、C组的b、c、d亚组在缺血前即刻持续输注异丙酚,停药时间为相应的再灌注时点。停药后即刻取肝组织,测定丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果与a亚组比较,各组的b、c、d亚组肝组织SOD活性降低,MDA含量升高(P<0.05或0.01)。与A组比较,B、C组的b、c、d亚组肝组织SOD活性升高, MDA含量降低(P<0.05或0.01),但B、C组间肝组织SOD、MDA水平差异无统计学意义(P>0.05)。结论异丙酚2、4 mg·h-1·100 g-1对大鼠缺血再灌注肝脏有一定的抗氧化作用。
Objective To observe the anti-oxidative effects of different doses of propofol on liver ischemia-reperfusion in rats. Methods Sixty Wistar rats were randomly divided into 3 groups with 20 animals in each group: control group; B group: propofol 2 mg · h -1 · 100 g -1; C group: propofol 4 mg · h-1 · 100 g-1, each subdivided into 4 subgroups, 5 in each subgroup, which were open only after anesthesia (a subgroup), reperfusion 30 min after ischemia for 30 min ); Ischemia 60min reperfusion 60min (c subgroup); ischemia 60min reperfusion 120min (d subgroup). Groups B, C, and C in group B continued propofol infusion immediately before ischemia, and the withdrawal time was the corresponding point of reperfusion. Liver tissue was taken immediately after stopping the treatment to determine the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). Results Compared with group a, the activity of SOD and the content of MDA in group b, c and d decreased (P <0.05 or 0.01). Compared with group A, the activity of SOD in group B, C and D in group B and group C increased and the content of MDA decreased (P <0.05 or 0.01), but there was no statistical difference in the level of SOD and MDA in group B and C Significance (P> 0.05). Conclusion Propofol 2,4 mg · h-1 · 100 g-1 has a certain anti-oxidative effect on the liver of ischemia-reperfusion rats.