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目的 探讨全腹内脏、下躯、下肢缺血 /再灌注损伤的病理生理学之动态变化规律。方法 ① 36犬随机分成 6组 ,分别于全腹内脏、下躯、下肢缺血 /再灌注损伤 0min、15min、2 5min、35min、45min和 6 0min获取同步血流动力学、血液生化、血气酸碱、血凝状态等指标以及EEG、RG与ECG指标 ,分析缺血 /再灌注损伤后各阶段多系统功能变化 ;② 12犬分别与全腹内脏、下躯、下肢缺血 /再灌注损伤 0min、15min、2 5min、35min、45min、6 0min后 ,获取心输出量、总外周阻力、右颈总及右股动脉血流量等指标 ,分析缺血 /再灌注损伤之后各阶段心血管系统功能变化 ;③从实验 1各组取 3犬获取肝、肾、胰、肠组织 ,另选 18犬随机分成 6组 ,取脑、肺、脊髓组织。分析缺血 /再灌注损伤之后各阶段多系统结构变化。结果 全腹内脏、下躯、下肢缺血 /再灌注损伤之模型可通过腹主动脉阻断方式制成。全腹内脏、下躯、下肢缺血 /再灌注损伤的全过程 ,按其血流动力学变化 ,可以分成低排高阻、高排低阻和低排低阻三期 ,该缺血 /再灌注损伤可造成多器官危象。结论 全腹内脏、下躯、下肢缺血再灌注损伤的发病机制主要与缺血 -应激、缺氧 -酸中毒、DIC -细胞毛细血管障碍三阶段有关。本实验尚提供了一个缺血 /再灌注损伤的动物实验模型
Objective To investigate the dynamic changes of pathophysiology of total abdominal viscera, lower body and lower limb ischemia / reperfusion injury. Methods ①Thirty-six dogs were randomly divided into 6 groups: synchronized hemodynamics, blood biochemistry, blood gas acid Alkaloids, blood coagulation status and other indicators of EEG, RG and ECG, analysis of multi-system changes in various stages of ischemia / reperfusion injury; ② 12 dogs were respectively impacted with ischemia / reperfusion injury of visceral, lower body and lower limbs , 15 min, 25 min, 35 min, 45 min and 60 min, cardiac output, total peripheral resistance, right common carotid artery and right femoral artery blood flow were measured to analyze the changes of cardiovascular system function at different stages after ischemia / reperfusion injury ; ③ From experiment 1, 3 dogs were taken from each group to obtain liver, kidney, pancreas and intestine tissues. The other 18 dogs were randomly divided into 6 groups. Brain, lung and spinal cord tissues were obtained. Multi-system structural changes at various stages after ischemia / reperfusion injury were analyzed. Results The models of total abdominal viscera, lower body and lower limb ischemia / reperfusion injury can be made by abdominal aorta occlusion. Whole abdominal viscera, lower body, lower extremity ischemia / reperfusion injury in the whole process, according to their hemodynamic changes, can be divided into low-row high resistance, high-row low resistance and low-row low resistance three, the ischemia / Perfusion injury can cause multiple organ crisis. Conclusion The pathogenesis of ischemia, reperfusion, hypoxia-acidosis and DIC-cell capillary barrier in the whole visceral, lower body and lower extremities is mainly related to the three stages. This experiment also provides an experimental animal model of ischemia / reperfusion injury