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铁和锌是水稻生长必需的微量元素,但在低洼或酸性土壤中,过量的二价铁和锌对水稻生长具毒害作用,最终造成生物量和产量下降。为探讨水稻苗期耐亚铁毒、锌毒的遗传机制,利用优质粳稻品种Lemont和高产籼稻品种特青为亲本构建的高代双向回交导入系和308个在染色体上均匀分布的SNP标记剖析耐亚铁毒、锌毒相关的QTL。从双向导入系共检测到42个影响耐亚铁毒、锌毒相关性状如苗高、苗干重、根干重以及胁迫与对照相对值的QTL,多数位点增强亚铁毒、锌毒抗性的有利等位基因来自Lemont。其中同时在2个背景下表达的QTL有4个,占定位QTL总数的9.52%,说明大多数QTL的表达具有明显的遗传背景效应。同一遗传背景下同时影响耐亚铁毒和锌毒的QTL有9个,其中QSdw5在2个背景中均被检测到,其效应大小和方向一致,说明水稻苗期耐亚铁毒、锌毒之间存在遗传重叠位点。因此,通过分子标记辅助选择从Lemont中导入或聚合有利的遗传重叠区域,可以提高特青对亚铁毒、锌毒的抗性水平。
Iron and zinc are essential trace elements for rice growth. However, excess iron and zinc in low or acid soils can have toxic effects on rice growth, resulting in a decrease in biomass and yield. In order to investigate the genetic mechanism of resistance to ferrous and zinc toxicity in rice seedling stage, a high-generation two-way backcross inbred line and 308 SNPs on chromosome were constructed by using high-quality japonica rice Lemont and high-yielding indica rice Teqing as parents Resistance to ferrous and zinc toxic QTLs. A total of 42 QTLs were detected that were resistant to ferrous and zinc poisoning, such as seedling height, seedlings dry weight, root dry weight and relative stress and control values, Sexually favorable alleles are from Lemont. Among them, four QTLs were expressed in two backgrounds at the same time, accounting for 9.52% of the total number of QTLs, indicating that most QTLs have significant genetic background effects. In the same genetic background, there were 9 QTLs that were resistant to both ferrous and zinc toxicity, of which QSdw5 was detected in 2 backgrounds and its effect was the same in size and direction, indicating that the resistance to ferrous and zinc toxicity There is a genetic overlap between sites. Therefore, through molecular marker-assisted selection, the favorable genetic overlap region of Lemont can be introduced or polymerized, which can increase the resistance of Teqing to ferrous and zinc poisoning.