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乳品生产企业和销售商们经常会遇到在液奶及乳制品中加入干酪乳清的问题。近年来,通过分析酪蛋白糖巨肽(GMP)的含量来确定乳中是否掺杂干酪乳清。非免疫法检测乳制品中GMP既昂贵又耗时,而且灵敏度低。目前,开发了一种新的夹心酶联免疫法(ELISA)实现了原料乳中乳清掺假问题的定性和定量检测;该方法以兔多克隆抗酪蛋白糖巨肽作为抗体,以已知浓度的液态干酪乳清(0.02%～20%)为标准品建立校正曲线。方法的检测限为0.047%(V/V),定量限为0.14%(V/V)。抗体具有专一性,与乳中其他成分(κ-酪蛋白除外)没有交叉反应,能够成功的用于乳制品中酪蛋白糖巨肽的检测。试验样品的检测回收率在95.62%～113.88%。批间和批内变异系数分别小于6%和7%。该抗体在3个月内,其准确度和重复性保持不变,并且可以随时取用。
Dairy producers and sellers often encounter the problem of adding cheese whey to liquid milk and dairy products. In recent years, the content of the casein glycomacropeptide (GMP) has been analyzed to determine whether the milk is doped with cheese whey. Non-immunoassay to detect GMP in dairy products is both expensive and time-consuming, with low sensitivity. Currently, a new sandwich enzyme-linked immunosorbent assay (ELISA) has been developed for the qualitative and quantitative detection of whey adulteration problems in raw milk. The method uses rabbit polyclonal anti-caspase-glycoprotein as an antibody, Concentration of liquid cheese whey (0.02% ~ 20%) as a standard to establish a calibration curve. The detection limit of the method was 0.047% (V / V) and the limit of quantification was 0.14% (V / V). Antibodies are specific and do not cross-react with other components in the milk (except for κ-casein) and are successfully used in the detection of casein glycomacropeptides in dairy products. The test sample recovery rate of 95.62% ~ 113.88%. Inter-and intra-batch CVs were less than 6% and 7%, respectively. The antibody in 3 months, its accuracy and repeatability remain unchanged, and can be readily available.