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了解中哈边境新疆艾比湖湿地游离蜱中斑点热群立克次体的感染情况。利用斑点热群立克次体外膜蛋白A(ompA)基因对艾比湖湿地游离亚洲璃眼蜱、边缘革蜱、血红扇头蜱进行PCR检测,并对阳性样本进行测序和BLAST序列分析,并应用Mega5.0软件建立分子系统进化树。结果中哈边境地区艾比湖湿地游离蜱斑点热群立克次体电泳阳性率36.84%(56/152),BLAST分析显示,艾比湖湿地游离蜱斑点热群立克次体基因型为Rickettsia raoultii,与从匈牙利患蜱传染的病人肿大淋巴结中分离获得的R.raoultii关系最近,处于同一分支(Gen Bank登录号为JQ798904)。结论是新疆艾比湖湿地游离蜱斑点热立克次体感染较为严重,存在立克次体自然疫源地,应尽快制定防制措施,以免危机动物及人类健康。
To understand the infection status of spotted fever group Rickettsia in ticks in the Aibi Lake wetland of China-Kazakhstan border. Using the ompA gene of the spotted heat group RAPD, PCR was performed on Eimeria tenella, Edgeworm and R. eryngii in Lake Aibi wetland. The positive samples were sequenced and analyzed by BLAST. Application of Mega5.0 software to establish molecular phylogenetic tree. Results The positive rate of free tick tick pygmy group was 36.84% (56/152) in the Lake Aibi wetland in China-Kazakhstan border area. The BLAST analysis showed that the Rickettsia genotype The raoultii, recently associated with R. raoultii isolated from swollen lymph nodes in patients infected with ticks in Hungary, is on the same branch (Gen Bank Accession No. JQ798904). The conclusion is that in Xinjiang Aibi Lake wetlands free tick tick hot rickettsia infection is more serious, there is a natural epidemic of rickettsial pathogens, prevention and control measures should be formulated as soon as possible in order to avoid the crisis of animals and human health.