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目的:探讨溃疡性结肠炎(Ulcerative Colitis,UC)中医症候类型与患者血清中黏膜地址素粘附分子1(Madcam1)、肿瘤坏死因子--α(TNF-α)及白介素-8(IL-8)表达水平的相关性,为UC的临床症候分型及分证论治提供依据.方法:通过收集南通市中医院门诊及住院的UC患者108例,同时选择20例正常人作为对照.观察UC患者临床表现及肠镜下结肠黏膜的病理形态,判断其病情的轻重.对收集的UC患者进行症候分型,分为大肠湿热证,脾虚湿热证,肝郁气滞证,寒热错杂证,脾肾阳虚证.采用双抗体夹心ELISA法检测各组UC患者血清中Madcam-1,TNF-a和IL-8的表达水平.通过统计学分析,探讨各项指标与UC症候类型的相关性.结果:中重度UC患者血清中IL-8、TNF-α、Madcam1蛋白水平明显高于轻度患者与正常人群,具有明显的相关性.大肠湿热证与脾虚湿热证UC患者血清中IL-8、TNF-α、Madcam1蛋白水平明显高于其他组与正常人群,具有明显的相关性.结论:检测UC患者血清中Madcam-1,TNF-a及IL-8的表达水平,可作为判断UC病情轻重及临床症候类型的依据,抗Madcam-1,TNF-a及IL-8抗体可能是治疗UC的有效方法.“,”Objectives:Studying the collection of Madcam-1,TNF-a and IL-8in the serum of patients with UC and its TCM syndrome types.Methods:108cases of UC patients were selected at outpatient and ward in Nantong Chinese Medicine Hospital which met the standard from June 2013 to Apri12016,20normal persons were involved for control.The UC patients were divided into mild activity,moderate activity and moderate to severe activity,and five TCM syndrome types,large intestine wet heat syndrome,dampness-heat due to spleen deficiency syndrome,hepatic stagnation and spleen deficiency type syndrome,intermingled heat and cold syndrome,Yang deficiency of spleen and kidney syndrome.Detected the level of TNF-a,IL-8and Madcamlin serum of UC patients and normal person.Summary of clinical collected set of data entry.The data were analyzed with SPSS13analysis software.Measurement data were analyzed using ANOVA analysis with mean±standard deviation.The comparison between groups variance by using the least significant difference method,using the hetogeneity of variance and Dunnett's t-test method,correlation analysis using pearson product moment correlation analysis,P＜0.05was considered statistically significant.Results:IL-8,TNF-a,Madcam-1 in the serum of patients with moderately and severely active UC were significantly higher than that of mild activity UC and normal persons (P＜0.05);IL-8,TNF-a,Madcam-1in the serum of UC patients with syndrome of large intestine wet heat syndrome and dampness-heat due to spleen deficiency significantly higher than that of other syndromes and normal persons (P＜0.05);There was distinct collection between differentiation of syndromes with IL-8,TNF-α,Madcam -1in the serum.Conclusion:Detecting serum Madcam-1,TNF-a and IL-8in UC patients maybe a good index for judging the severity and TCM syndrome of UC;Anti Madcam-1,TNF-a and IL-8antibody maybe useful treatment for UC.