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目的 研究HBV不同感染状态对CCL2 0表达水平的影响,探讨CCL2 0在乙肝病毒感染中的作用。方法 通过基因体外转染技术,建立HBV不同感染状态的肝细胞模型;以内对照定量RT PCR检测HBV不同感染状态下CCL2 0的表达水平。结果 HBV不同感染状态下CCL2 0的表达水平不同,HBV未感染肝细胞CCL2 0表达水平每10 6 细胞为(2 .6 5±0 .0 2 )pg 10 μl,HBV短暂感染肝细胞CCL2 0的表达水平每10 6 细胞为(3.4 3±0 .0 2 )pg 10 μl,而HBV持续感染肝细胞CCL2 0的表达水平每10 6 细胞为(1.2 2±0 .0 4 )pg 10 μl,上述三种不同感染状态下肝细胞表达CCL2 0的水平差异有统计学意义,表现为HBV短暂感染肝细胞>未感染肝细胞>HBV持续性感染肝细胞(P <0 .0 5 )。在HBV同一感染状态下,CCL2 0的表达水平与HBV感染时间、细胞的培养时间、病毒载量等因素未见显著性相关;上述细胞经乙酸肉豆蔻佛波醇(PMA)活化后,相应细胞CCL2 0表达均明显增加(P <0 .0 5 ) ,但并不改变CCL2 0在各细胞中的表达格局。结论 HBV的不同感染状态影响了CCL2 0的表达。
Objective To investigate the effect of different HBV infection status on the expression of CCL20 and to explore the role of CCL20 in hepatitis B virus infection. Methods The hepatocyte model with different infection status was established by gene transfection in vitro. The expression of CCL20 in different infection status of HBV was detected by internal control quantitative RT-PCR. Results The expression levels of CCL20 in different HBV infected states were different. The expression levels of CCL20 in HBV non-infected hepatocytes were (2.56 ± 0.22) pg / 10 6 cells per 10 6 cells, and the transient infection of hepatocytes with CCL20 The expression of CCL20 per 106 cells was (3.4 3 ± 0. 0 2) pg 10 μl, while that of HBV persistent infection CCL 2 0 was (1.2 2 ± 0. 0 4) pg 10 6 cells per 10 6 cells. The levels of CCL20 expression in hepatocytes under three different infection states were statistically significant, showing a transient infection of HBV with hepatocytes> uninfected hepatocytes> persistent HBV infected hepatocytes (P <0.05). In the same infection status of HBV, the expression level of CCL20was not significantly correlated with HBV infection time, cell culture time, viral load and other factors; after the cells were activated by PMA, the corresponding cells CCL20 expression was significantly increased (P <0.05), but does not change the CCL20 expression pattern in each cell. Conclusion The different infection status of HBV affects the expression of CCL20.