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目的探讨类风湿关节炎(RA)患者易感性与HLA-DR基因的相关性。方法研究对象为75例RA患者及40例健康体检者,采用PCR-SSP法检测HLA-DR4和HLA-DR53基因分型、BNII全自动特定蛋白分析系统速率散射比浊法和乳胶凝集法同时定量和定性检测类风湿因子,并对结果进行回顾性分析。结果①RA患者HLA-DR4和HLA-DR53等位基因发生的频率数分别为44%和56%,与对照组比较均P<0.01,结果有非常显著性意义。组间比较,P>0.05结果无显著性意义,其DR基因与RA的相对危险率分别为3.70和2.64,HLA-DR4高于HLA-DR53。②75例RA患者其速率比浊法和乳胶凝集法分别有59例阳性和44例阳性,两法经χ2检验,结果均P<0.01,有非常显著性意义;组间比较经χ2检验,P<0.05有显著性意义说明速率比浊法好于乳胶凝集法。③75例类风湿性关节炎患者速率比浊法检测结果为198.59±379.21IU/ml,同对照组比较经t检验,P<0.01结果有非常显著性意义。结论RA患者其HLA-DR基因与RA的发生易感性明显有关,为临床诊断和鉴别诊断提供依据。
Objective To investigate the association between susceptibility to HLA-DR gene and rheumatoid arthritis (RA). Methods The subjects were 75 RA patients and 40 healthy subjects. The genotypes of HLA-DR4 and HLA-DR53 were detected by PCR-SSP, and the BNII automated specific protein assay system was used for the simultaneous quantification of rate of turbidity and latex agglutination And qualitative detection of rheumatoid factor, and the results of a retrospective analysis. Results ① The frequencies of HLA-DR4 and HLA-DR53 alleles in RA patients were 44% and 56% respectively, which were all significantly lower than those in control group (P <0.01). The results were highly significant. There was no significant difference between the two groups (P> 0.05). The relative risk of DR gene and RA was 3.70 and 2.64, respectively, and HLA-DR4 was higher than that of HLA-DR53. ② The rate of turbidimetry and latex agglutination in 75 patients with RA were positive in 59 and positive in 44, respectively. The two methods were tested byχ2 test, the results were all P <0.01, with significant significance. Comparing the two groups by χ2 test, P < 0.05 significant significance rate turbidimetric method is better than latex agglutination. ③ The rate turbidimetric test results of 75 patients with rheumatoid arthritis were 198.59 ± 379.21 IU / ml, compared with the control group by t test, P <0.01 results have a very significant significance. Conclusion The HLA-DR gene is significantly associated with the susceptibility to RA in RA patients, providing a basis for clinical diagnosis and differential diagnosis.