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植物根际细菌对植物的生长和健康具有非常积极的作用,尤其是对处于营养贫瘠等逆境下的沙漠植物,研究细菌菌群组成是理解植物生长促进根际细菌(PGPR)与植物互作关系并应用于提高植物生产力的前提和基础。基于16S rDNA基因的文库构建和分析,本文对比分析了浑善达克沙漠中褐沙蒿根际细菌群组成及其随季节的变化情况,探讨了PGPR在褐沙蒿生长发育过程中的作用和功能。结果表明:浑善达克沙漠中的褐沙蒿根际细菌类群主要有10类,即Firmicutes、Verrucomicrobia、Gemmatimonadetes、Acidobacteria、Actinobacteria、Bacteroidetes、Chloroflexi和α-、β-、δ-proteobacteria;在褐沙蒿生命活动相对旺盛的4月份和8月份最优势的类群是属于α-proteobacteria的具有固氮功能的细菌和具有降解大分子物质并兼有生防功能的Sphingomonadales菌群;而在褐沙蒿代谢相对弱的时期(11月份),根际细菌多样性反而高于植物代谢强的时期(4月份和8月份)。本研究提示我们,在挖掘PGPR资源时应该注意选择合适的植物生长时期。
Plant rhizobacteria have a very positive effect on the growth and health of plants, especially for desert plants under the stress of nutrient deficiency. Studying the bacterial flora is to understand the relationship between plant growth and plant rhizobacteria (PGPR) And applied to improve plant productivity on the premise and foundation. Based on the library construction and analysis of 16S rDNA gene, this paper comparatively analyzed the composition of rhizosphere bacteria in the rhizosphere of Artemisia mandshurica in the Hunshandake Desert and its change with season, and discussed the role of PGPR in the growth and development of Artemisia annua and function. The results showed that there are 10 main bacterial groups in the rhizosphere of the brown sand Artemisia of Hunshandake desert, namely Firmicutes, Verrucomicrobia, Gemmatimonadetes, Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi and α-, β- and δ-proteobacteria. The most dominant taxa of April and August in Artemisia annua were nitrogen-fixing bacteria belonging to α-proteobacteria and Sphingomonadales bacteria having degrading macromolecules and biocontrol function. In the relative metabolism of Artemisia annua Weak period (November), rhizosphere bacterial diversity is higher than the period of strong plant metabolism (April and August). This study suggests that we should pay attention to choosing the appropriate plant growth period when mining PGPR resources.