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为探讨星形胶质细胞在缺血性神经元损伤中的作用及其与神经元损伤的关系 ,本实验阻塞大鼠大脑中动脉 2 h,再灌流0 .5～ 48h建立短暂局灶性脑缺血模型 ,进行 H-E染色 ;通过胶质原纤维酸性蛋白和细胞核增殖抗原免疫组化单重或双重反应 ,TU NEL和胶质原纤维酸性蛋白免疫组化双重反应观察了神经元和星形胶质细胞的反应。结果表明 :再灌流 2 4h缺血区面积最大 ,再灌流 6h开始出现神经元不可逆变性 ,2 4h梗塞成熟 ;星形胶质细胞表现为反应性、营养不良性和退形变三种不同的形态特点。再灌流 48h时星形胶质细胞数量开始增多。 48h之内星形胶质细胞无增生 ,且有少量星形胶质细胞凋亡。这些结果提示脑缺血时星形胶质细胞反应与神经元损伤密切相关 ,反应性星形胶质细胞是其积极应答神经元损伤的结果 ,在维持神经元存活中起作用。
In order to explore the role of astrocytes in ischemic neuronal injury and its relationship with neuronal damage, the experiment blocked the middle cerebral artery in rats 2 h, reperfusion 0.5 ~ 48h to establish transient focal brain Ischemia model and HE staining. Through the immunohistochemical single or double reaction of glial fibrillary acidic protein and nuclear proliferating antigen, TU NEL and glial fibrillary acidic protein immunohistochemical double reaction observed neurons and astrocytes Response of the cells. The results showed that the ischemic area was the largest at 24 h after reperfusion, irreversible neuronal degeneration began at 6 h after reperfusion, and infarcted at 24 h. Astrocytes showed three morphological features of reactivity, malnutrition and degeneration . At 48h after reperfusion, the number of astrocytes began to increase. Within 48h no proliferation of astrocytes, and a small amount of astrocyte apoptosis. These results suggest that astrocyte responses during cerebral ischemia are closely related to neuronal damage. Reactive astrocytes are the result of their positive response to neuronal damage and play a role in the maintenance of neuronal survival.