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用超临界CO_2萃取海洋微藻中的EPA和DHA，选用乙酸乙酯为改性剂，通过正交试验确定了工作压力、温度、CO_2消耗量和改性剂添加量等工艺条件的最优化组合，对日本小球藻Chlorellahiralaii、钝顶螺旋藻Spirulinaplatensis和亚心形扁藻Platymonassubcordiformis进行提取试验，并与直接酯化法、Bligh－Dyer法、索氏抽提法和乙醇－乙烷法等溶剂法作对比。以提取效率最高的直接酯化法为基础，超临界萃取法对日本小球藻中ω－3脂肪酸二十碳五烯酸（EPA）的提取率为92．1％，二十二碳六烯酸（DHA）为89．4％，对钝顶螺旋藻和亚心形扁藻的提取率也达到90％左右，高于其他溶剂法，产物中EPA和DHA的纯度亦优于溶剂法。
The extraction of EPA and DHA from marine microalgae by supercritical CO 2 and the use of ethyl acetate as modifier, the optimum combination of working conditions such as working pressure, temperature, CO 2 consumption and modifier addition were determined by orthogonal experiments , Chlorellahiralaii, Spirulinaplatensis and Platymonassubcordiformis of Chlorella were studied and compared with solvent methods such as direct esterification, Bligh-Dyer, Soxhlet extraction and ethanol-ethane method For comparison. Based on the direct esterification method with the highest extraction efficiency, the extraction rate of omega-3 fatty acid eicosapentaenoic acid (EPA) in Chlorella japonicus was 92.1% by supercritical fluid extraction, The acid (DHA) was 89.4%, and the extraction rate of Spirulina platensis and Platymonas subcordata was about 90% higher than other solvent methods. The purity of EPA and DHA in the product was also better than the solvent method.