近年来,蔬菜的农药残留问题成为大家关注的焦点问题,农药残留快速检测工作尤为重要。其中,酶抑制法因其较高的专一性、灵敏性、准确性及低成本等优点,在农残快速检测中被广泛应用。基于此,介绍酶抑制率法农残速测技术原理、方法和实际操作中常遇到的问题及解决办法。 In recent years, the issue of vegetable pesticide residues has become the focus of everyone’s attention, rapid detection of pesticide residues is particularly important. Among them, the enzyme inhibition method is widely used in the rapid detection of pesticide residues because of its high specificity, sensitivity, accuracy and low cost. Based on this, we introduce the principle, method and practical problems of pesticide residue rate measurement by enzyme inhibition rate method and their solutions.