论文部分内容阅读
以 6 0 mg/ L的 6 -甲氨基嘌呤 (简称 6 - DMAP)诱导栉孔扇贝三倍体时 ,在荧光显微镜下观察受精卵的染色体行为以及核相组成 ,对照三倍体率和孵化率 ,探讨了不同减数分裂期 6 - DMAP处理对染色体倍数性的影响。结果表明 ,处理 5～ 15min可见 3种类型图像 ,第 1种图像可见第一极体和一个雌性及雄性原核 ;第 2种图像可见第一极体和两个雌性原核及一个雄性原核 ;第 3种图像可见两个雌性原核和一个雄性原核 ,随着处理时间延长雌雄原核逐渐形成泡状结构。 6 - DMAP抑制第二极体产生的三倍体主要来源于发育至第 2次减数分裂中期的受精卵 ,使一个双倍性的雌性原核与一个雄性原核结合形成三倍体 ;受精卵在第 2次减数分裂的后期时接受处理 ,可使部分受精卵的两个雌性原核和一个雄性原核在融合和分离过程中发生异常。实验证明 ,6 - DMAP有效抑制了栉孔扇贝受精卵的染色体分离和原核移动 ,并可造成染色质分散 ,从而使极体排放受阻诱导出三倍体
In 6 0 mg / L of 6 - methylamino purine (referred to as 6 - of DMAP) upon induction scallop triploid, fertilized eggs were observed under a fluorescent microscope and nuclear chromosome behavior phase control triploid and hatchability The effects of 6 - DMAP at different stages of meiosis on chromosome doubling were discussed. The results showed that three types of images were seen after 5 ~ 15 min, the first polar body and one female and male pronucleus were seen in the first image, the first polar body and two female pronucleus and one male pronucleus were seen in the second image, The images show two female pronuclei and one male pronucleus, and the prolactinoma gradually forms bubble-shaped structures as the treatment time prolongs. 6 - The triploid produced by DMAP inhibition of the second polar body mainly originated from the fertilized egg which developed into the second metaphase, and one double viroid was combined with one male pronucleus to form the triploid. At the second meiotic metaphase, the two female pronuclei and one male pronucleus were abnormally fused and separated during the late treatment of meiosis. Experiments show that 6 - DMAP can effectively inhibit chromosome segregation and prokaryotic movement of fertilized eggs of Chlamys farreri, and can cause chromatin dispersion, resulting in inhibition of polar body induced triploid