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目的:探讨单纯疱疹病毒I型(HSV-1)感染后在宿主骨髓是否形成潜伏感染。方法:尾静脉注射HSV-1感染小鼠,分别于病毒接种后5、15、30 d处死实验组和对照组小鼠,取股骨分离培养原代骨髓间充质干细胞(BMSCs),并对其进行成骨细胞诱导和树突状细胞诱导的鉴定,PCR法检测骨髓及BMSCs中HSV-1特异条带。结果:BMSCs成骨诱导碱性磷酸酶和钙结节染色均呈阳性,培养出形态典型的树突状细胞。各实验组骨髓及其对应的BMSCs的第1、2代均检测到HSV-1的特异DNA片段。结论:骨髓是HSV-1形成潜伏感染的靶器官之一,且能随细胞增殖而持续携带HSV-1。
Objective: To investigate whether latent infection occurs in the host bone marrow after herpes simplex virus type 1 (HSV-1) infection. Methods: Mice infected with HSV-1 were injected intravenously into the tail vein and the mice were killed at 5, 15 and 30 days after virus inoculation. Primary bone marrow mesenchymal stem cells (BMSCs) Osteoblast induction and dendritic cell induction were performed. HSV-1 specific bands in bone marrow and BMSCs were detected by PCR. Results: BMSCs were positive for osteogenic induction of alkaline phosphatase and calcium staining. The typical dendritic cells were cultured. The HSV-1 specific DNA fragments were detected in the first and second generation of bone marrow and its corresponding BMSCs in each experimental group. CONCLUSION: Bone marrow is one of the target organs for latent infection of HSV-1 and can carry HSV-1 continuously with cell proliferation.